安徽省汉族人群Miltenberger血型调查分析  

The investigation and analysis of Miltenberger blood group in Anhui Han population

作　　者：周娟[1] 吕蓉[2] 朱帮强[3] 韩婷婷[1] 刘忠[1,4] 

机构地区：[1]安徽医科大学公共卫生学院,合肥230032 [2]安徽省血液中心输血研究室,合肥230031 [3]安徽医科大学第一附属医院输血科,合肥230022 [4]中国医学科学院输血研究所,成都610052

出　　处：《安徽医科大学学报》2014年第3期313-315,共3页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81370669)

摘　　要：目的调查安徽省Miltenberger血型的分布情况,为建立红细胞库奠定基础。方法随机采集安徽省血液中心2 660例非血缘关系汉族无偿志愿献血者外周血样乙二胺四乙酸(EDTA)抗凝血标本,并提取DNA。采用序列特异性引物PCR(PCR-SSP)方法对Miltenberger血型系统进行检测,并通过血清学及测序方法验证。结果 2 660例献血者中有24例PCR-SSP阳性,经血清学及测序确认均为GP.Mur表型。结论在安徽省汉族人群中,GP.Mur表型的频率为0.9%。目前还未发现Miltenberger血型系统的其他表型。Objective To study the distribution of Miltenberger blood group in Anhui province to establish the red cell bank. Methods Peripheral blood samples anticoagulated with ethylene diamine tetraacetic acid（ EDTA） from 2 660 unrelated volunteer Han blood donors were randomly collected, and genomic DNA were extracted. PCR-Se-quence specific primers（ PCR-SSP） was used to screen for Miltenberger blood group, and serological and DNA se-quencing method was used for validation. Results 24 donors were positive by PCR-SSP, and they were GP. Mur phenotype by serological and DNA sequencing method. Conclusion The frequency of GP. Mur phenotype is 0.9%in Anhui Han population. Other phenotypes in Miltenberger blood group system have not yet been found in Anhui Han population.

关 键 词：血型 Miltenberger血型 Mur抗原 

分 类 号：R457.11[医药卫生—治疗学]