T细胞亚群与慢性丙型肝炎病毒相关性分析  

Study on the relationship between T cell subsets and count and virus load in patients with hepatitis C

作　　者：殷先尧 郑美娟[1] 徐元宏[1] 

机构地区：[1]安徽医科大学第一附属医院检验科,合肥230022 [2]肥西县中医院检验科,肥西231200

出　　处：《安徽医科大学学报》2014年第3期327-330,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81302525)

摘　　要：目的探讨外周血中T淋巴细胞亚群与慢性丙型肝炎(CHC)患者丙型肝炎病毒(HCV)复制程度的关系。方法采用流式细胞仪直接免疫荧光法检测69例CHC患者、20例正常健康者外周血中的T细胞亚群;利用荧光定量PCR法检测患者血清HCV RNA。结果①CHC患者CD4+T细胞百分率[(42.87±6.11)]及CD4+/CD8+比值[(1.34±0.25)]明显低于正常健康者[(49.55±6.68)]和[(1.82±0.11)](P<0.01),而CD8+T细胞百分率[(32.78±5.48)]则高于正常健康者[(27.35±4.32)](P<0.01),CD3+T细胞百分率差异无统计学意义(P>0.05)。②随着HCV RNA复制载量的增加,CD8+T细胞百分率逐渐增加,而CD4+T细胞百分率及CD4+/CD8+比值逐渐降低。结论 CHC患者外周T淋巴细胞亚群比例改变,可能是HCV感染慢性化的重要原因之一。随着HCV RNA表达水平的升高,可能是导致T淋巴细胞应答能力下降的重要原因。Objective To investigate the correlation between T cell subsets and HCV viralload in HCV infection. Methods Flow cytometry was used to detect peripheral T cell subsets count of 69 patients with hepatitis C and 20 cases of normal health human, and fluorescent quantitative PCR was used to detect viral load in patients with hepa-titis C. Results ① The study showed that the percentage ratio of CD4 ＋T cells （42.87 ±6.11） and of CD4 ＋/CD8 ＋（1.34±0.25） in patients patients was significantly lower than those of normal health group,it was 49.55±6.68 and 1.82±0.11 （P〈0.01）;but the percentage ratio of CD8 ＋T cells （32.78±5.48） was higher than that of the normal health human （ 27.35 ±4.32 ） （ P〈0.01 ） . There were no significant differences between the two groups in the percentage ratio of CD3 ＋ T cells. ② The viral load gradually increased as the percentage ratio of CD8 ＋T cells increased, while the percentage ratio of CD4 ＋T cells and the percentage ratio of CD4 ＋T cells and CD4 ＋/ CD8 ＋ were decreased gradually. Conclusion It may be one of the important reasons as the chronic infec-tion of hepatitis C virus by change of T cell subsets, and may be an important cause leading to the decrease of T lymphocyte response ability as the expression level of HCV RNA increases.

关 键 词：HCV RNA T淋巴细胞亚群 相关性 

分 类 号：R392.11[医药卫生—免疫学]