NEDD4-1基因在病理性瘢痕中的表达及意义  

Expression of NEDD4-1 gene and its significance in pathological scar

作　　者：张锦松[1] 朱飞[1] 胡超[1] 张胜权[2] 杨森[3] 

机构地区：[1]安徽医科大学第一附属医院整形外科,合肥230022 [2]安徽医科大学生物化学教研室,合肥230032 [3]安徽医科大学第一附属医院皮肤科,合肥230022

出　　处：《安徽医科大学学报》2014年第3期347-350,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30973124)

摘　　要：目的研究NEDD4-1基因在正常皮肤与增生性瘢痕和瘢痕疙瘩中的表达情况,探讨其在病理性瘢痕发病机制中的作用。方法收集临床标本30例,其中增生性瘢痕10例(A组)、瘢痕疙瘩10例(B组)、正常皮肤10例(C组),采用Western blot法和普通RT-PCR方法检测A、B、C 3组中NEDD4-1蛋白和mRNA表达情况,并进行统计学分析。结果在A、B组中,NEDD4-1 mRNA和蛋白均可表达,但表达差异无统计学意义(P>0.05),两组分别与C组比较,差异无统计学意义(P>0.05)。结论 NEDD4-1基因在病理性瘢痕中均可表达,但NEDD4-1的表达与病理性瘢痕的发生机制之间的关系仍需进一步研究。Objective To examine the gene expression of NEDD4-1 in pathological scar and explore its effect on＆amp;nbsp;formation of pathological scar. Methods Tissue specimens were collected from 10 cases of hypertrophic scar （ group A） , 10 cases of keloid （ group B） and 10 cases of normal skin （ group C） respectively. Western blot and RT-PCR techniques were performed to detect the protein and gene expression of NEDD4-1 . Results NEDD4-1 mRNA and protein in both group A and B could be expressed, while the difference between the two groups was not significant （P〉0.05）. Moreover, compared with the group C, the difference in gene expression of NEDD4-1 did not reach statistical significance in both group A and group B （P〉0.05）. Conclusion In our experimental study, NEDD4-1 gene is present in pathological scar, but further studies in the detailed mechanism are warranted.

关 键 词：GWAS NEDD4-1 病理性瘢痕 瘢痕疙瘩 

分 类 号：R392.11[医药卫生—免疫学]