模拟肺纤维化过程中Caveolin-1表达及其临床意义  

Change of Caveolin-1 in the process of pulmonary fibrosis model in vitro an in vivo and its clinical significance

作　　者：张明明[1] 吕长俊[1] 张晓荣[1] 许玲[1] 李洪波[1] 王晓芝[1] 赵洪文[2] 

机构地区：[1]滨州医学院附属医院呼吸内科,滨州256600 [2]中国医科大学附属第一医院呼吸疾病研究所,沈阳110000

出　　处：《临床与实验病理学杂志》2014年第3期270-274,278,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：滨州医学院科技计划(BY2010KJ015);山东省教育厅高等学校科技计划(J12LM63);山东省"泰山学者"岗位基金资助

摘　　要：目的探讨TGF-β1对成纤维细胞小凹蛋白1(Caveolin-1,Cav-1)表达的影响,进一步探讨不同亚组成纤维细胞Cav-1基础表达的差异及肺纤维化模型不同时期Cav-1的表达。方法 Western blot法检测不同浓度TGF-β1对成纤维细胞作用不同时间后Cav-1表达的影响,流式细胞分选后用Western blot法检测不同亚组成纤维细胞中Cav-1表达的差异,免疫组化法检测博莱霉素(bleomycin)对大鼠肺纤维化模型中不同时期Cav-1的表达的影响。结果 Cav-1的表达呈时间和剂量依赖性降低,Thy-1+亚组Cav-1的基础表达高于Thy-1-亚组;随着纤维化的进展,Cav-1表达呈下降趋势。结论 Cav-1表达降低可能与肺纤维化有关,Thy-1-亚组对致纤维化刺激的高反应性可能与Cav-1低表达有关。旨在恢复或增加Cav-1生物活性的治疗措施可能有助于恢复上皮细胞的正常再生过程和延缓纤维化的进展。Purpose To determine the expression of Caveolin-1 （Cav-1） in NIH3T3 fibroblasts under the influence of profibroblastic factor TGF-β1.Methods The expression of Cav-1 under the influence of TGF-β1 at different time and concentration were analyzed by Western blot.Basal level of Cav-1 in different subsets after fluorescence activated cell sorting was also investigated by Western blot,the expression of Cav-1 in different phases of bleomycin induced rat fibrosis model was analyzed by immunohistochemical methods.Results The expression of Cav-1 increased in a time and dose dependent manner,Cav-1 basal level was higher in Thy-1 ＋ subset than that in Thy-1-subset.The expression of Cav-1 decline as the process of fibrosis aggravated.Conclusion Low expression of Cav-1 was probably related to the progress of pulmonary fibrosis,the high responsibility of Thy-1-fibroblast subset to profibroblastic stimulus was probably related to its low expression of Cav-1.Treatment methods aiming at restoring and enhancing the bioactivity of Cav-1 may restore the normal process of regeneration of the epithelial cells and delay the progression of fibrosis.

关 键 词：肺纤维化 小凹蛋白1 THY-1 免疫组织化学 

分 类 号：R563.9[医药卫生—呼吸系统]