小麦抗病基因Lr46/Yr29/Pm39、Sr2/Yr30和Lr68的遗传特性及其与主要农艺性状的关联分析  

Genetic Characteristics of Wheat Resistance Gene Lr46/Yr29/Pm39,Sr2/Yr30and Lr68 and Association Analysis of Main Agronomic Traits

作　　者：阳霞[1] 刘路平[1] 孙道杰[1] 张玲丽[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100

出　　处：《西北植物学报》2014年第3期454-462,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金："十二五"农村领域国家科技计划(2012AA101105);美国唐仲英育种基金;"十二五"国家科技支撑计划(2013BAD01B02)

摘　　要：利用与抗病基因Lr46/Yr29/Pm39、Sr2/Yr30和Lr68等紧密连锁的分子标记,对小麦品种(系)‘RL6077’和‘西农979’构建的BC1F1和F2群体进行抗病基因遗传分析,结合主要农艺性状分析,探究小麦抗病基因Lr46/Yr29/Pm39、Sr2/Yr30和Lr68的遗传特性及其与主要农艺性状的关联性;并对检测的聚合有3个抗慢锈病基因(Lr68+Sr2/Yr30+Lr46/Yr29/Pm39)位点的聚合体进行SSR标记遗传背景回复率检测。结果表明:(1)F2群体中Lr68基因的传递率(65.41%)比理论值(75%)偏低,Lr46/Yr29/Pm39基因和Sr2/Yr30基因的传递率(分别为75.83%、74.53%)与理论值(75%)相符合;BC1F1群体中Lr68基因的传递率(44.27%)比理论值(50%)偏低,Lr46/Yr29/Pm39基因和Sr2/Yr30基因的传递率(分别为56.64%、55.11%)均比理论值(50%)偏高。(2)Lr46/Yr29/Pm39和Sr2/Yr30基因位点均与株高、穗长、穗下茎长、穗下节长呈极显著正相关关系;Lr68和Sr2/Yr30基因位点均与穗粒数呈显著或极显著正相关关系;Lr46/Yr29/Pm39基因位点与千粒重呈显著负相关、与单株成穗数呈显著正相关关系。(3)对BC1F1群体中聚合有Lr68+Sr2/Yr30+Lr46/Yr29/Pm39基因位点的92个聚合体的遗传背景回复率检测发现,轮回亲本‘西农979’遗传背景回复率最高达91.67%,其中遗传回复率达90%以上的聚合体有3株,占回交群体(655株)总体的0.46%。该研究结果为优异抗病基因资源‘RL6077’的进一步利用提供了理论参考,对创制小麦多种抗病新种质具有重要意义。We used close linkage molecular markers of resistance genes Lr46/Yr29/Pm39, Sr2/Yr30 and Lr68 to analysis the genetic characteristics of the resistance genes in the BC1 F1 and F2 descendant popula- tions. The BC1 F1 and F2 descendant populations both came from ＇ RL6077 ＇ and ＇ Xinong 979 ＇. Combined with the main agronomic traits, we could deeply comprehend the gene heredity rule of wheat disease resist- ance genes as well as the correlation with agronomic traits. We also evaluated the background response rates of 92 plants in the BC1F1 population who have three kinds of resistance genes at the same time. The result showed that：（1）In the F2 population the transfer rate ot gene Lr68（65.41%）, but the transfer rate of Lr46/Yr29/Pm39 （75.83%） and Sr2/Yr30 （74.53%） is consistent with the theo- retical,respectively. In the BC1 F1 population, the transfer rate of gene Lr68 （44.27 %） transferred lower than theory,while the transfer rate of Lr46/Yr29/Pm39 （56.64%） and Sr2/Yr30 （55.11%） gene deliv- ered higher than theory,respectively. （2）All these three kinds of resistance genes have extremely signifi- cant positive correlation with the plant height, spike length, spike-stalk and spike-internode. The Lr68 gene and Sr2/Yr30 gene have significant or extremely significant positive correlation with the spike grain num- ber. The Lr46/Yr29/Pm39 gene has significantly negative correlation with the thousand kernel weight and significantly positive correlation with the ear number per plant. （3）In the evaluate of the background re- sponse rates of plants in the BC1F1 population which have Lr46/Yr29/Prn39,Sr2/Yr30 and Lr68 genes. Meanwhile,we can find that the biggest response rate of recurrent parent ＇Xinong 9797 was up to 9t. 67%. There were three plants whose response rates exceed 90% and they take 0.46% of the BC1F1 popula- tion. This study provided an important theoretical reference to using the excellent genetic resources of ‘RL6077’. It also has important significance to c

关 键 词：小麦 抗病基因 遗传特性 农艺性状 关联分析 

分 类 号：Q789[生物学—分子生物学]