基于ITS序列对栽培中国樱桃遗传多样性及其群体遗传结构的分析  

Genetic Diversity and Population Genetic Structure among Local Chinese Cherry Varieties[Cerasus pseudocerasus(Lindl.)G.Don]Based on ITS Sequence

作　　者：何文[1] 张静[1] 黄智林[1] 陈清[1] 汤浩茹[1] 汤福义[1] 王小蓉[1,2] 

机构地区：[1]四川农业大学园艺学院,四川雅安625014 [2]四川农业大学果蔬研究所,成都611130

出　　处：《西北植物学报》2014年第3期463-472,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(31272134);四川省教育厅重大培育专项(2011A005)

摘　　要：该研究基于ITS序列对栽培中国樱桃[Cerasuspseudocerasus（Lindl．）G．Don]18个群体共154个个体的遗传多样性及其群体遗传结构进行了分析，以期从DNA序列水平上揭示栽培中国樱桃种质资源的遗传背景，为保护和利用中国樱桃种质资源提供理论依据。结果表明：（1）154条ITS序列比对后共定义了11个单倍型，表现出较低的遗传多样性（h=0．5590，π=0．0012），群体间遗传多样性也表现出较大差异（h=0～0．9050，π=0～0．0061）。（2）群体分析显示，群体间遗传分化水平较低（Fst-0．1400），只有14％的遗传变异来自群体间，而86％的遗传变异来自群体内部。研究认为，栽培中国樱桃在驯化过程中所产生的奠基者效应以及瓶颈效应可能是导致群体遗传多样性丢失的主要原因，而较长的世代周期及较短的分化时间可能导致了群体间低的遗传分化；因此，对栽培中国樱桃种质应采取就地保护策略；若需迁地保护种质建议减少采样群体数而增加群体内个体数量的采样策略。In order to effectively preserve, utilize and reveal the genetic background of Chinese cherry [Cera- sus pseudocerasus （Lind1.） G. Don],we analyzed the genetic diversity and population genetic structure a- mong 18 populations （total 154 individuals） based on ITS sequence. The results showed that： （1）11 haplo- types were yielded based on the alignment of 154 ITS fragments,indicating a low level of haplotypes and nuclear diversity （h=0. 559 0,π=0. 001 2） with high variation among populations （h=0～0. 905 0,π=0～ 0. 006 1）. （2）Fairly low level of genetic differentiation （FsT=0. 140 0） was found among these popula- tions, with only 14% of total variability from among populations and 86% of genetic variation within groups. These results suggested that founder and bottleneck effect produced by domestication and recent reduction of population probably affected the genetic diversity. The long alternation of generation and shorthistory of differentiation may lead to a low genetic differentiation among populations. According to these results,a conservational plan for sampling or preserving fewer populations but more individuals from each population for the species was proposed.

关 键 词：中国樱桃 地方种质 ITS序列 遗传多样性 群体遗传结构 

分 类 号：Q789[生物学—分子生物学]