白菜型冬油菜不育系LRCMS花器生理生化特性及其雄蕊发育特征研究  

Physiobiochemical Characteristics and Stamen Development Characteristics of LRCMS Flower in Winter Rapeseed(Brassica campestris)

作　　者：鲁美宏 孙万仓[1,2,3] 孔德晶[1,2,3] 杨宁宁 刘自刚[1,2,3] 

机构地区：[1]甘肃省油菜工程技术研究中心 [2]甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室 [3]甘肃农业大学农学院,兰州730070

出　　处：《西北植物学报》2014年第3期509-515,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：农业部公益性行业(农业)科研专项(200903002-04);国家高技术研究发展计划(2011AA10A104);现代农业产业技术体系建设专项(CARS-13)

摘　　要：以白菜型冬油菜‘陇油8号’ב陇油9号’杂交后代中选育出的不育系LRCMS，以及代表性甘蓝型油菜不育系（J39A、J68A、PollOA）和它们的保持系为材料，研究LRCMS在盛花期花蕾和花中POD、s0D、CAT活性和可溶性蛋白含量、过氧化物酶（POD）同工酶谱变化特征及其雄蕊败育过程。结果显示：（1）所有参试材料花朵中POD活性普遍高于花蕾。（2）不育材料POD活性高于保持材料，甘蓝型不育材料POD活性较保持材料增加幅度大于LRCMS与‘陇油9号’，LRCMS花蕾与花的POD活性分别较保持系材料‘陇油9号’高出15．0％和9．0％；不育材料花的CAT活性高于保持系材料89％～293％，而其花蕾的CAT活性低于保持系材料25％～43％，LRCMS与其它不育材料间无显著差异；不育系材料花SOD活性低于保持系材料15％～25％，LRCMS与其它不育材料无显著差异，其花蕾SOD活性高于保持材料6％～112％，但LRCMS增加幅度低于其它不育材料；保持系材料花蕾与花中蛋白质含量为0．014～0．020mg／g，不育系材料花蕾与花中蛋白质含量为0．012～0．016mg／g，所有不育材料蛋白质含量均低于保持材料。（3）LRCMS的POD同工酶谱在花中较对应的可育系‘陇油9号’和其它甘蓝型雄性不育多1条迁移率（Rf）为0．623的条带。（4）LRCMS雄蕊败育起始于造孢细胞期，造孢细胞粘连，无核仁，绒毡层未发育，为无花粉囊型不育。研究表明，LRCMS与其他类型油菜不育系有着不同的遗传背景，是一个新的白菜型冬油菜不育类型。The LRCMS is a cytoplasmic male sterile material selected from the off springs of ‘Longyou 8＇ × ‘Longyou 9＇. POD, SOD, CAT activities and soluble protein content of LRCMS in flowering buds and flowers,peroxidase （POD） isozyme changes during the abortion process were investigated. The results showed that. （1）POD activity of all the tested materials in flower is generally higher than that of buds. （2） Sterile material POD activity is higher than that of maintainer line. Compared with the maintainer line, the increase of POD activity in kale sterile material is greater than that of LRCMS and ‘Longyou 9 ’. POD ac-tivities in LRCMS flowers and buds are 15.0% and 9.0% higher than that of the maintainer line. CAT ac- tivity in sterile material flowers is higher than that of maintainer line （89%～293% higher） ,while CAT ac- tivity in buds is lower than that of the maintainer line （25%～43% lower）;and there is no significant difference between LRCMS and other sterile materials. SOD activity in sterile material flowers is lower than that of the maintainer line,while SOD activity in buds is higher than that of the maintainer line （6%～112% higher） ,and the increase in LRCMS is lower than that of other sterile materials. Flowers SOD activi- ty is 15%～25% lower than that of the maintainer line and there is no significant difference between LRC- MS and other sterile materials. Buds of the maintainer line and their protein content is 0. 014 ～ 0. 020 mg/g,while the protein content in buds of the sterile material is 0. 012～0. 016 mg/g. Protein contents of all sterile materials are lower than that of the maintainer line. （3）POD isoenzyme test indicated an Rf value of 0. 623 band in flower of LRCMS which is missing in other male sterile lines and fertile line ‘Longyou 9 ’. （4）LRCMS sterile stamens starting at the sporogenous cell,sporogenous cell adhesion,nuclear-free ker- nel,tapetum absence. The study find that LRCMS and other CMS have different genetic backgrounds. It is

关 键 词：冬油菜 细胞质不育 生理生化 雄蕊发育 

分 类 号：Q945.6[生物学—植物学] Q944.58