转基因产品检测标准物质的定值和不确定度研究进展  

Research Progress on Value Characterization and Uncertainty Evaluation of Reference Materials for Genetically Modified Organisms

作　　者：张丽[1,2] 吴刚[1] 武玉花[1] 沈平[3] 宋贵文[3] 周云龙[3] 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物生物学重点开放实验室,武汉430062 [2]中南民族大学生命科学学院,武汉430074 [3]农业部科技发展中心,北京100122

出　　处：《农业生物技术学报》2014年第3期362-371,共10页Journal of Agricultural Biotechnology

基　　金：国家转基因生物新品种培育重大专项(No.2013ZX08012-003)和No.2013ZX08012-005)

摘　　要：转基因产品检测标准物质(genetically modified organisms detection reference materials,GMOs detection RMs)是转基因生物(genetically modified organisms,GMOs)安全监管、转基因产品定性与定量检测、检测方法研究与标准化工作中必不可少的物质基础。为了解决我国转基因产品检测标准物质匮乏的问题,我国启动了"转基因产品检测标准物质研制"研究课题,旨在借鉴欧盟和美国等研制转基因产品检测标准物质的先进经验,建立适合中国国情的转基因产品检测标准物质研制体系,以满足我国转基因生物安全性评价和监管的需求。本研究针对国际上转基因产品检测基体、基因组DNA和质粒DNA不同类型标准物质的研制流程与量值表达方式进行概述,阐述了不同类型标准物质量值不确定度的评定方式,分析目前转基因产品检测标准物质研制中存在的问题,为我国转基因产品检测标准物质的研制提供参考资料,为标准物质的定值和不确定度的评定提供理论依据。Genetically modified organisms detection reference materials（GMOs detection RMs） used in genet- ically modified organisms （GMOs） detection are essential for GMOs safety supervision, qualitative and quanti- tative detection. To date, only a few institutions, such as EU Joint Research Centre （Institute for Reference Ma- terials and Measurements, IRMM） and American Oil Chemists＇ Society （AOCS）, have developed certified ref- erence material （CRM） for GMOs testing. In China, research and development of GMOs detection RM are still at the initial stage. Based on the price of CRMs on GMOs testing is very expensive, and the available of CRMs for GMOs can not meet requirements in China, the funding agency in China supported a research proj- ect named ＂Study on RMs for GMOs detection＂, which aims to develop CRMs for GMOs detection used in Chinese GMOs safety supervision requirements. This paper presented overview on production process and val- ue characterization pattern of current existing RMs based on matrix, genomic DNA and plasmid DNAs. We al- so addressed the problems in development of RMs of GMOs detection in this paper. This review can provide a useful reference for production of Chinese RMs of GMOs detection, and theoretical basis for value characterization and uncertainty evaluation of RMs.

关 键 词：转基因生物 检测 标准物质 定值 不确定度 

分 类 号：Q781[生物学—分子生物学]