新疆转基因抗虫棉组织中Bt杀虫蛋白表达的研究  

The Study of Bacillus Thuringiensis Insecticidal Protein Expressed in the Tissue of Xinjiang Transgenic Insect Resistant Cotton

作　　者：孙严[1] 孟庆玉[1] 王冬梅[1] 危晓薇[1] 美丽古力[1] 

机构地区：[1]新疆农科院核技术生物技术研究所，新疆 乌鲁木齐 830051

出　　处：《新疆农业大学学报》2000年第3期24-26,共3页Journal of Xinjiang Agricultural University

基　　金：国家"九五"科技攻关项目(95-001-02-01);国家高技术发展计划项目(95-001-02-02)

摘　　要：利用酶联免疫吸附测定法 (ELISA)结合卡那霉素涂棉花的方法 ,检测转基因抗虫棉组织中Bt杀虫蛋白的表达。这一检测方法不仅可以快速、方便地对转基因抗虫棉组织中Bt杀虫蛋白表达量进行定性、定量测定 ,也对转基因抗虫棉的选育和抗虫性测定提供了依据。Based on the reaction among antibody,antigen and enzyme antibody,an enzyme linked immunosorben assay (ELISA) method was deveploped to detect the Bacillus thuringiensis (Bt) insecticidal protein expressed in the tissue of Bt transgenic insect resistant cotton,and with the Kanamycin detecting method. This method may be used for rapid and sffective detection of Bt imseceticidal protein expressed in Xinjiang transgenic insect resistant cotton,and it can offer the basis for detecting bollworm resistance and selecting transgenic cotton stranis.

关 键 词：BT杀虫蛋白 转基因抗虫棉 基因表达 棉花 

分 类 号：S562.035[农业科学—作物学]