基于线粒体nad1和nad4基因对四川省多头带绦虫种群遗传多样性的分析  

Population genetic diversity of Taenia multiceps isolated from Sichuan Province based on mitochondrial nad1and nad4genes

作　　者：郝桂英[1,2] 杨应东[3] 周英姿[4] 杨光友[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]西昌学院,四川西昌615013 [3]攀枝花市农林科学研究院畜牧水产所,四川攀枝花617061 [4]眉山市畜牧局,四川眉山620010

出　　处：《中国兽医科学》2014年第3期251-257,共7页Chinese Veterinary Science

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)

摘　　要：为探讨多头带绦虫的种群遗传多样性，应用PCR方法扩增20株分离自四川省攀枝花市、雅安市、成都市的山羊脑部和肌间的多头蚴的线粒体nadl基因和nad4基因的部分序列，并进行分析。序列分析结果显示，20株多头蚴的pnadl基因和pnad4基因序列分别有2个和8个变异位点，长度分别为753bp和801bp，变异率分别为0～0．3％和0～0．8％，检测出3个和6个单倍型，总的单倍型多样性分别为0．574±0．055和0．742±0．074，核苷酸多样性分别为0．00082±0．00053和0．00288±0．001，平均遗传距离分别为0．002和0．003。构建的种系发育树显示，四川分离株与已知多头带绦虫位于同一分支，且与分布的地理区域、寄生部位没有直接的相关性。结果表明，四川多头带绦虫的遗传多样性水平低，其中nadl基因的遗传多样性水平低于nad4基因的遗传多样性。The aim of this study was to determine the level of population genetic diversity of Taenia multiceps. Partial sequences of the mitochondrial had1 gene and nad4 gene of 20 isolates of coenurus col- lected from the brain or intramuscular of goats in Panzhihua, Ya＇ an and Chengdu prefectures of Sichuan Province were amplified and analyzed. In result, there were 2 variable sites and 8 variable sites in the pnadl and pnad4 sequences,the mitochondrial genes sequenced in this study were 753 bp for pnadl and 801 bp for pnad4 in length,and the sequence variations of pnadl gene and pnad4 gene were from 0 to 0.3 % and from 0 to 0.8 %,respectively. Three haplotypes and 6 haplotypes were detected in pnadl gene and pnad4 gene,with the global haplotype diversity being 0. 574± 0. 055 （pnadl gene） and 0. 742± 0. 074 （pnad4 gene）. The nucleotide diversities were 0.000 82±0. 000 53 for pnadl gene and 0. 002 88--0. 001 for pnad4 gene, and the average genetic distances were 0. 002 for pnadl gene and 0. 003 for pnad4 gene, respectively. The phylogenetic trees showed that all the 20 isolates from Sichuan Province and other T. multiceps iso- lates available in GenBank were clustered in the same clade, and there was no direct correlation between parasitic sites and geographical regions. The results indicated that the level of genetic diversity of T. multiceps from Sichuan Province was low,and the diversity of nadl gene was lower than that of had4 gene.

关 键 词：多头带绦虫 nad1基因 nad4基因 遗传多样性 四川省 

分 类 号：S852.734[农业科学—基础兽医学]