深Ⅱ度烫伤早期犬血液生理指标及四种细胞因子质量浓度的动态变化  

Dynamic changes of blood physiologic index and levels of 4cytokines in serum and tissue of dogs at the early stage of deep partial-thickness burn

作　　者：余树民[1] 白欣[1] 左之才[1] 彭西[1] 王娅[1] 邓俊良[1] 甘梦[1] 

机构地区：[1]四川农业大学动物医学院环境公害与动物疾病四川省高校重点实验室,四川雅安625014

出　　处：《中国兽医科学》2014年第3期308-312,共5页Chinese Veterinary Science

基　　金：国家自然科学基金面上项目(31172379);四川省学术与技术带头人培养基金项目;四川省教育厅重点资助项目(08ZA055)

摘　　要：以中华田园犬为研究对象，用99口C多层纱布覆盖犬腰荐部多个面积为3cm。区域皮肤，制备深Ⅱ度烫伤模型，采集烫伤前和烫伤后第6、12、24和48小时血样和烫伤皮肤组织样品，检测血液生理指标和细胞因子IL-6、IL-8、IL-10、TNF-α的质量浓度。结果显示，烫伤后犬血液白细胞、中性粒细胞和单核细胞的浓度均显著升高（P〈0．05），烫伤后第24小时达到峰值，淋巴细胞的浓度显著降低（P〈0．05），烫伤后第12小时降至最低；犬血清和组织中IL-6、IL-10和TNF-α的质量浓度在烫伤后第6～24小时均显著高于烫伤前（P〈0．05），烫伤前后血清中IL-8的质量浓度未发生显著变化（P〉0．05），组织中IL-8的质量浓度烫伤后第24小时显著升高（P〈0．05）。结果表明，深Ⅱ度烫伤早期（48h以内）试验犬呈现全身性炎性反应特征，血清和烫伤组织中促炎／抗炎细胞因子水平不同程度升高。The present study aimed to observe the dynamic changes of blood physiologic index and cy- tokines in serum and scalded tissue of dogs at the early stage of deep partial-thickness burn. Chinese rural dogs were used to produce the deep partial-thickness burn model by multilayer pledgets at 99℃ overlap- ping the lumbar-sacral skin with 3 cm2 area for 23 s, thus the blood samples were taken at the different stage of pre- and at 6 h,12 h,24 h and 48 h post-scald,in order to detect blood physiology index with auto- matic Blood Cell Counter, and the levels of cytokines IL-6,IL-8,IL-10 and TNF-α in serum and scalded tissue with ELISA kits. In result, the counts of WBC,GRA and MID significantly increased in post-scalded canine blood compared with that in the pre-scald canine blood（P〈0.05） ,and reached the maximum at 24 h post-scald,while LYM＇s counts significantly decreased（P〈0.05） and reached at the minimum at 12 h post-scald. In the experimental period from 6 h to 24 h post-scald, the levels of IL-6, IL-10 and TNF-α sig- nificantly increased in both serum and tissue scalded（P〈0.05）. The level of IL-8 in serum showed no sig- nificant difference between pre- and post-scald（P〉0.05）,but the level in the tissues was significantly higher 24 h post-scald than those at other time points （P 〈 0. 05）. These results suggested that the experimental dogs presented characterization of systemic inflammatory response at the early stage of deep partial-thickness burn（within 48 h）, and the pro-/anti-inflammatory cytokines increased to different extents in serum and the scalded tissues.

关 键 词：犬 深Ⅱ度烫伤 血液生理指标 细胞因子 

分 类 号：S858.292[农业科学—临床兽医学]