类肝素酶在人类早期复发性自然流产中的作用研究  

Association of levels of heparanase in the villi with human recurrent spontaneous abortion

作　　者：穆雅琴[1] 刘润花[1] 朱壮彦[1] 刘丽华[1] 孟燕[1] 赵富玺[1] 

机构地区：[1]山西大同大学医学院免疫学研究所,大同037009

出　　处：《现代免疫学》2014年第2期99-102,共4页Current Immunology

基　　金：山西省青年科学基金资助项目(2010021036-4);国家人口计生委资助项目(C-73);国家自然基金资助项目(81170621/H0425)

摘　　要：本研究探讨类肝素酶(heparanase,HPSE)与人类早期复发性自然流产的关系,进一步研究HPSE在自然流产发生过程中的作用机制。本研究收集非意愿性正常妊娠者和早期复发性自然流产者绒毛组织标本,采用实时荧光定量RT-PCR和免疫组织化学法检测其HPSE mRNA和蛋白的表达情况。实验结果显示:正常妊娠组的HPSE mRNA和蛋白表达率均明显高于复发性自然流产组,差异有统计学意义(P<0.05)。以上结果提示:在正常妊娠过程中HPSE表达增高,可以促进正常胚泡的植入过程,而复发性自然流产中HPSE表达降低会影响胚泡的植入,导致自然流产的发生。To investigate the relationship between heparanase （HPSE） and human recurrent spontaneous abortion, we collected the villi from normal pregnant women and patients with recurrent spontaneous abortion. By using real-time fluorescent quanti- tative reversed transcript PCR and immunohistochemical methods we determined the expression of HPSE mRNA and protein. The results indicated that the expressions of both HPSE mRNA and protein in the normal group were significantly increased in comparison with those in the recurrent spontaneous abortion group （P^0.05）. These results revealed that HPSE promotes implantation of the embryo and participates in the procedure of normal pregnancy, and lower expression of HPSE may lead to recurrent spontaneous abortion.

关 键 词：复发性自然流产 HPSE 实时荧光定量RT-PCR 免疫组化 

分 类 号：R714.21[医药卫生—妇产科学]