肠菌群转化的吴茱萸提取物抗肿瘤活性分析  

Study on anti-tumor activity of Fructus evodiae extracts transformed by intestinal bacteria

作　　者：韩洁[1] 田维毅[1] 

机构地区：[1]贵阳中医学院基础部微生物教研室,贵阳550002

出　　处：《现代免疫学》2014年第2期133-135,共3页Current Immunology

基　　金：2006年贵州省科技厅攻关项目[NY字(2006)3047]

摘　　要：为研究吴茱萸受肠菌群体外转化后提取物的抗肿瘤活性。本文利用人粪便肠菌群转化吴茱萸提取液,分别用HPLC和MTT法对转化前后吴茱萸水提液中的吴茱萸碱含量和对KB(人口腔表皮样癌细胞)的抗肿瘤作用进行检测和分析。结果表明,经肠菌群转化后提取液中吴茱萸碱的含量逐渐增加,抗肿瘤能力也随着含量的增加而增加,并在转化后24h吴茱萸碱的抗肿瘤能力达到最大,肿瘤抑制率最高可达73.5%。本研究结果为筛选和利用肠道菌株转化吴茱萸组分提供参考。In order to analyze the effects of in vitro transformation by intestinal bacteria on the Fructus evodiae medicinal ingre- dients and its anti-tumor activity, the Fructus evodiae extracts transformed or not, were analyzed by HPI.C and the anti-tumor ability to KB （human oral epithelial-like carcinoma） was observed by MTT method. The results showed that the content of evodiamine and the anti-tumor activity were gradually increased after transformation. The maximize rate of tumor inhibition （ up to 73.5 % ） occurred within 24 h of transformation. It is important for further study on screening and utilizing these bacteria strains,

关 键 词：吴茱萸碱 肠菌群 生物转化 高效液相色谱 

分 类 号：R93[医药卫生—生药学]