甾体激素受体辅激活子对子宫内膜异位症患者异位子宫内膜基质细胞中基质细胞衍生因子1表达的影响  

Expression of stromal cell-derived factor-1 in endometriosis by steroid receptor coactivatorson steroid hormone

作　　者：施秀[1] 徐炜[1] 孙莹[1] 戴辉华[1] 王秀丽[1] 

机构地区：[1]南京医科大学第一附属医院妇科,210036

出　　处：《中华妇产科杂志》2014年第2期114-119,共6页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金（81070471）;江苏省医学重点人才建设和培养计划（RC201161）

摘　　要：目的 探讨子宫内膜异位症（内异症）患者异位子宫内膜（异位内膜）基质细胞中甾体激素受体辅激活子（SRC）和基质细胞衍生因子1（SDF-1）的表达水平变化,了解不同类型SRC对雌二醇和孕酮调控的异位内膜基质细胞SDF-1表达的影响.方法 2010年5月至2012年10月,选择在南京医科大学第一附属医院妇科因卵巢内异症囊肿[美国生育学会修订的内异症分期标准（r-AFS）分期为Ⅲ～Ⅳ期]接受手术治疗,术后标本经病理检查证实为内异症的16例患者的异位内膜（增殖期9例,分泌期7例）及同期因非经期放置宫内节育器的无内异症病史且月经周期正常的健康妇女10例的正常内膜（增殖期5例,分泌期5例）.所有患者术前3个月内均未服用过促性腺激素释放激素激动剂（GnRH-a）类药物.采用实时荧光定量PCR技术比较正常内膜和异位内膜在不同月经周期中SRC-1、SRC-2、SRC-3和SDF-1αmRNA表达水平.异位内膜基质细胞分离、培养、传代后,分别用含有10-8 moL/L的17β-雌二醇或10出mol/L的17β-雌二醇＋10-6 mol/L孕酮的培养液继续培养.分别于培养24、48、72、96 h后收集上清液,采用ELISA法检测上清液中SDF-1α的蛋白表达水平.脂质体法分别介导SRC-1和SRC-2的小分子干扰RNA（siRNA）转染异位内膜基质细胞,转染后2d换用分别含有10-8mol/L的17β-雌二醇或10-8moL/L的17β-雌二醇＋10-6mol/L孕酮的培养液继续培养3 d,ELISA法检测上清液中SDF-1α的蛋白表达水平.结果 （1）周期性变化：正常内膜中,SRC-1、SRC-2和SDF-1α的表达均具有周期性变化,增殖期mRNA的表达水平分别为5.6±1.2、3.8±1.1、2.7±0.5,分泌期的表达水平分别为2.6±1.0、2.1±1.0、1.6±0.5；分别比较,差异均有统计学意义（P＜0.05）；异位内膜缺乏周期性变化.（2）雌、孕激素培养不同时间后SDF-1α蛋白表达水平：17β-雌二醇培养异位内膜基质细胞48、72和96 h后,SDF-1αObjectives To study the expression patterns of steroid receptor coactivators （SRC） and steroid-induced stromal cell-derived factor-1 （SDF-1） in endometriosis,and to explore the roles of SRC in the steroid-induced SDF-1 expression endometriosis.Methods From May 2010 to October 2012,16 endometriosis cases at stages Ⅲ or Ⅳ according to the revised American Society for Reproductive Medicine classification undergoing surgery in the First Affiliated Hospital to Nanjing Medical University were enrolled in this study.Their ectopic endometrium were from ovarian endometriomata which were identified pathologically with 9 cases at proliferative phase and 7 cases at secretory phase.The normal endometrium were acquired from the healthy women with normal menstrual cycle （n =10,proliferative phase =5,secretory phase =5）.The mnRNA levels of SRC and SDF-1α during the menstrual cycle were detected by quantitative real-time polymerase chain reaction.Ectopic endometrium stromal cells were purified and cultured in medium containing 17β-estradiol （10-8mol/L） or 17β-estradiol （10-8 mol/L） ＋ progesterone （10-6 mol/L）.At 24,48,72 and 96 hours,the supernatants were collected to measure SDF-1α expression by ELISA.Ectopic endometrium stromal cells were transfected respectively with siRNA of SRC-1 and SRC-2 using lipofectamine.Two days after transfection,17β-estradiol （10-8 moL/L） or 17β-estradiol （10-8 mol/L） ＋ progesterone （10-6 mol/L） were added into the media.On the third day after the steroid hormones treatment,the media were collected to quantify SDF-1α expression with ELISA.Results （1） Cyclical changes： the SRC-1,SRC-2 and SDF-1 α showed marked cyclic differences in normal endometrium （P ＜ 0.05）.In proliferative phase and secretory phase,the SRC-1,SRC-2 and SDF-1 α were 5.6 ± 1.2,3.8 ± 1.1,2.7 ± 0.5 and 2.6 ± 1.0,2.1 ± 1.0,1.6-± 0.5,respectively.There was no periodic variation in the expression of SRC-1,SRC-2 and SDF-1α in ectopic endometrium throughout the menstru

关 键 词：子宫内膜异位症 核受体共激活因子1 核受体共激活因子2 趋化因子CXCL12 

分 类 号：R711.71[医药卫生—妇产科学]