Molecularly Imprinted Polymers for Selective Extraction of Crystal Violet from Natural Seawater coupled with HighPerformance Liquid Chromatographic Determination  

作　　者：LIAN Ziru WANG Jiangtao 

机构地区：[1]Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education,Ocean University of China [2]Marine College,Shandong University at Weihai

出　　处：《Journal of Ocean University of China》2014年第2期236-242,共7页中国海洋大学学报（英文版）

基　　金：supported by the Natural Science Foundation of China (41076065);the Major State Basic Research Development Program of China (973 Program) (2010CB428701)

摘　　要：Molecularly imprinted polymers （MIPs） were prepared by the bulk polymerization using crystal violet as the template molecule, and the methacrylic acid and ethylene glycol dimetheacrylate as functional monomer and cross-linker, respectively. Sys- tematic investigations of synthetic conditions were conducted. The surface morphology and recognition mechanism of the obtained polymers were studied using scanning electron microscope and spectrophotometric analysis. MIPs showed high atTmity to template molecule and were successfully applied as special solid-phase extraction sorbent for selective extraction of crystal violet from natural seawater. An off-line molecularly imprinted solid-phase extraction （MISPE） method followed by high-performance liquid chroma- tography with diodearray detection for the analysis of crystal violet was also established. MISPE columns have good recoveries for crystal violet standard solutions and good linearity was obtained over the concentration range of 0-200 ~tg L-1 （R2 〉 0.99）. Finally, two natural seawater samples were investigated. The recoveries of spiked seawater on the MISPE columns were from 44.47% to 62.34%, the relative standard deviation （n=3） being in the range of 2.89%-5.96%.Molecularly imprinted polymers(MIPs) were prepared by the bulk polymerization using crystal violet as the template molecule, and the methacrylic acid and ethylene glycol dimetheacrylate as functional monomer and cross-linker, respectively. Systematic investigations of synthetic conditions were conducted. The surface morphology and recognition mechanism of the obtained polymers were studied using scanning electron microscope and spectrophotometric analysis. MIPs showed high affinity to template molecule and were successfully applied as special solid-phase extraction sorbent for selective extraction of crystal violet from natural seawater. An off-line molecularly imprinted solid-phase extraction(MISPE) method followed by high-performance liquid chromatography with diodearray detection for the analysis of crystal violet was also established. MISPE columns have good recoveries for crystal violet standard solutions and good linearity was obtained over the concentration range of 0-200 μg L-1(R2 > 0.99). Finally, two natural seawater samples were investigated. The recoveries of spiked seawater on the MISPE columns were from 44.47% to 62.34%, the relative standard deviation(n=3) being in the range of 2.89%-5.96%.

关 键 词：crystal violet molecularly imprinted polymer ADSORPTION solid-phase extraction natural seawater 

分 类 号：X834[环境科学与工程—环境工程] O657.72[理学—分析化学]