Comparison of the Respiratory Metabolism of Juvenile Litopenaeus vannamei Cultured in Seawater and Freshwater  

作　　者：DING Sen WANG Fang DONG Shuanglin LI Ying 

机构地区：[1]The Key Laboratory of Mariculture of Ministry of Education of China,Ocean University of China [2]Laboratory of Riverine Ecological Conservation and Technology,Chinese Research Academy of Environmental Sciences

出　　处：《Journal of Ocean University of China》2014年第2期331-337,共7页中国海洋大学学报（英文版）

基　　金：supported by National Program on Key Basic Research Project (Grant No.2009CB118706)

摘　　要：Litopenaeus vannamei, a euryhaline species, can be cultured at a wide range of salinities. The emergence of freshwater pond-culture ofL. vannamei is an important prelude to the continued development of shrimp culture in China. In this study, we com- pared the respiratory metabolism of juvenile L. vannamei cultured in freshwater and saltwater by measuring their oxygen consump-tion rate （OCR）, ammonium-type nitrogen excretion rate （AER） and pyruvate kinase （PK） and lactate dehydrogenase （LDH） activi-ties at different molting stages in order to physiecologically characterize juvenile L. vannamei under freshwater conditions. The re- suits showed that OCR was significantly higher in saltwater than in freshwater at all stages of molting cycle. However, variation of OCR among molting stages in saltwater was similar with that in freshwater, and the highest OCR was observed at post-molting stage. At all stages of molting cycle, AER was significantly higher in freshwater than in saltwater, and the highest was observed at post-molting stage. The activity of PK was significantly higher in saltwater than in freshwater. Conversely, the activity of LDH was higher in freshwater than in saltwater in general. Significant variation of PK and LDH activities in molting cycle was observed in saltwater and freshwater. The results indicated that aerobic metabolism of juvenile L. vannamei was more active in saltwater than in freshwater; while its protein metabolism was more active in freshwater than in saltwater.Litopenaeus vannamei, a euryhaline species, can be cultured at a wide range of salinities. The emergence of freshwater pond-culture of L. vannamei is an important prelude to the continued development of shrimp culture in China. In this study, we compared the respiratory metabolism of juvenile L. vannamei cultured in freshwater and saltwater by measuring their oxygen consumption rate(OCR), ammonium-type nitrogen excretion rate(AER) and pyruvate kinase(PK) and lactate dehydrogenase(LDH) activities at different molting stages in order to physiecologically characterize juvenile L. vannamei under freshwater conditions. The results showed that OCR was significantly higher in saltwater than in freshwater at all stages of molting cycle. However, variation of OCR among molting stages in saltwater was similar with that in freshwater, and the highest OCR was observed at post-molting stage. At all stages of molting cycle, AER was significantly higher in freshwater than in saltwater, and the highest was observed at post-molting stage. The activity of PK was significantly higher in saltwater than in freshwater. Conversely, the activity of LDH was higher in freshwater than in saltwater in general. Significant variation of PK and LDH activities in molting cycle was observed in saltwater and freshwater. The results indicated that aerobic metabolism of juvenile L. vannamei was more active in saltwater than in freshwater; while its protein metabolism was more active in freshwater than in saltwater.

关 键 词：Litopenaeus vannamei saltwater FRESHWATER respiratory metabolism molt stage 

分 类 号：S968.22[农业科学—水产养殖]