蓝莓H34组培快繁技术  

A study on tissue culture and rapid propagation of blueberry ‘H34'

作　　者：姜丽琼[1] 肖前刚[1] 李文俊[1] 谢文娟[1] 徐志萍[1] 陈文灵[1] 

机构地区：[1]成都市农林科学院,成都611130

出　　处：《林业科技开发》2014年第2期85-88,共4页China Forestry Science and Technology

基　　金：成都市2012年重点科技基金支持项目(编号:2012-013)

摘　　要：对H34优良蓝莓品种的优良单株进行外植体诱导、继代增殖、生根培养和炼苗移栽试验研究。通过研究，筛选出H34蓝莓品种最佳诱导培养基：改良WPM＋6-BA1．0mg／L＋NAA0．5mg／L；增殖培养基：改良WPM＋6-BA0．1-0．2mg/L＋NAA0．05～0．1mg/L；生根培养基：改良WPM＋ABT 0．6mg／L＋IBA0．6mg／L，移栽最佳基质：腐殖土，成活率可达92．06％。In this paper,superior individuals selected from super blueberry‘H34'were used as study materials for explant induction,subculture,culture of rooting and transplanting. The results showed that the optimum medium for explant induction of blueberry‘H34'was: modified WPM + 6-BA 1. 0mg / L + NAA 0. 5mg / L. Proliferation medium was: the improved WPM + 6-BA 0. 1- 0. 2 mg / L + NAA 0. 05- 0. 1 mg / L. Rooting culture medium was: the improved WPM + ABT 0. 6 mg / L + IBA 0. 6 mg / L. The optimum transplanting matrix was humus soil,and the seedling survival rate in which reached 92. 06%.

关 键 词：蓝莓 组织培养 外植体诱导 继代增殖 生根诱导 炼苗移栽 

分 类 号：S796[农业科学—林木遗传育种]