介入法建立比格犬和小型猪急性心肌梗死模型的对比研究  

The comparison of establishment of the acute myocardial infarction model between the beagle dogs and the mini-pigs by interventional technique

作　　者：顾晓龙[1] 黄军[1] 董正华[1] 雷辉燕[2] 龚志华[3] 文艳飞[3] 向定成[3] 

机构地区：[1]南方医科大学研究生学院,广州510515 [2]广州军区总医院超声影像科,广州510010 [3]广州军区总医院心血管内科,广州510010

出　　处：《重庆医学》2014年第9期1090-1092,共3页Chongqing medicine

摘　　要：目的比较介入法建立比格犬与小型猪两组动物急性心肌梗死模型的成功率及风险,为选择更合适的动物建模提供理论基础。方法比格犬和五指山小型猪各6头,全身麻醉下行右侧股动脉切开穿刺术,左冠状动脉造影后选择冠状动脉球囊送至前降支中段行缺血预适应,并持续封堵180min后撤出球囊,术前、术后均连续观察心电图变化,术后7d处死动物取出心脏行病理学检查。结果比格犬组均存活,小型猪组死亡1例(1/6);比格犬组发生心肌梗死1例(1/6),小型猪组5例(5/6);比格犬组未发生恶性心律失常,小型猪组6例均发生恶性心律失常(6/6);两组造模所需时间比较[(4.61±0.47)h vs.(4.82±0.55)h],差异无统计学意义(P>0.05)。结论小型猪在介入法建立心肌梗死模型中虽然风险高于比格犬,但是成功率仍较高,是建模的理想动物。Objective To explore the success rate and the risk of establishment of the acute myocardial infarction model between the beagle dogs and the mini-pigs by interventional technique ,further to provid theoretical basis for choose a more suitable animal model .Methods 6 dogs and 6 mini-pigs were anaesthetized ,then underwent the coronary arteriography via femoral artery .After is-chemic preconditioning the coronary balloon was inflated to occlude the middle left anterior descending coronary for 180 minutes . The electrocardiogram was examined throughout the operation and the pathological sections were examined until the animals were executed one week later .Results All beagle dogs survived ,while 1 case of mini-pigs dead（1/6） .There was 1 cases（1/6） of beagle dogs had acute myocardial infarction ,while 5（5/6）cases in mini-pigs .All mini-pigs had malignant arrhythmia（6/6） but never seen in beagle dogs .The time needed for building a model was similar between the two groups ,the difference had no statistical signifi-cance（P＆gt;0 .05） .Conclusion The risk of establish myocardial infarction model in mini-pigs is higher than beagle dogs ,but the suc-cess rate is still high ,it might be the better choice .

关 键 词：心肌梗死 猪  雏型 模型  动物 

分 类 号：R542.22[医药卫生—心血管疾病]