辣椒疫霉产毒缺陷与抗药性突变体筛选及其遗传特性  

SELECTION OF TOXIN-DEFICIENT AND FUNGICIDE RESISTANCE MUTANTS AND THEIR GENETICS OF PHYTOPHTHORA CAPSICI

作　　者：谢丙炎[1] 朱国仁[1] 吴新平[2] 顾宝根[2] 刘乃炽[2] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]农业部农药检定所,北京100026

出　　处：《植物保护学报》2000年第3期243-248,共6页Journal of Plant Protection

基　　金：国家"九五"科技攻关项目(96-005-01-12)

摘　　要：采用MNNC诱变与自交(S_1～S_3)纯合的方法,从辣椒疫霉的游动孢子群体中,筛选出1株带有抗霜脲氰标记的产毒缺陷突变体、2株抗甲霜灵的产毒突变体。产毒缺陷菌株和产毒菌株均对茄门甜椒致病,但产毒缺陷菌株的致病力降低一半,证明辣椒疫霉的致病显症过程与毒素作用有关,病菌毒素是重要的致病因子。此外,突变菌株的抗药性和产毒缺陷表型在无性游动孢子后代和有性卵孢子后代群体中均可稳定遗传。在姐妹配对F_2代卵孢子群体中,辣椒疫霉毒素产生和对甲霜灵的抗性为不完全显性基因所控制,而对霜脲氰的抗性为完全显性基因所控制。A toxin-deficient and cymoxanil-resistant mutant, two toxin-producing and metalaxyl-re-sistant mutants were obtained by mutagenesis of N-methyl-N'-nitrosoguanidine(MNNG) and self-hybrids from Phytophthora capski. The pathogenesis of toxin-deficient mutant to pepper was tested and but declined for 50% .Therefore, we could come to the conclusion that toxin was one of the most important pathogenic factors. The results also suggested that phenotypes of fungicide-resistant and toxin-deficient mutants of Phytophthora capski could be steadily inherited among zoospore and oospore populations. Incomplete dominant genes controlled the toxin production and the resistance to meta-laxyl,while resistance to cymoxanil was cotrolled by complete dominant gene.

关 键 词：辣椒疫霉 毒素 杀菌剂 抗性 突变体遗传 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]