PM_(2.5)急性暴露对运动即刻及24h恢复后大鼠TNF-α、hs-CRP、CC16的影响  

Effect of PM_(2. 5) exposure on serum TNF-α,hs-CRP and CC16 of the rats treated immediately or 24 hours later after exhaustive exercise

作　　者：李峰[1] 石辉[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《环境化学》2014年第3期375-380,共6页Environmental Chemistry

基　　金：青年科技基金(QN1043)资助

摘　　要：采用一次性递增负荷跑台运动对不同浓度PM2.5染毒大鼠进行训练,通过测定含量的变化,研究运动、PM2.5染毒及24 h恢复对大鼠血清肿瘤坏死因子-α(Tumor Necrosis Factor,TNF-α)、超敏C反应蛋白(hypersensitive C-reactive protein,hs-CRP)、克拉拉细胞蛋白(Clara Cell Protein,CC16)的影响机制,选取雄性SD大鼠64只(每组8只),随机分为运动即刻组(EC)、24 h恢复组(ECR)、7.5 mg·kg-1(LPE)、15 mg·kg-1(MPE)、30 mg·kg-1(HPE)、7.5 mg·kg-1+24 h恢复组(LPER)、15 mg·kg-1PM2.5+24 h恢复组(MPER)、30 mg·kg-1+24 h恢复组(HPER).采用气管滴注法PM2.5染毒然后进行一次性递增负荷跑台进行训练,运动即刻组与恢复组分别于训练后即刻及恢复24 h后处死,提取血清后通过酶联免疫法(Enzymelinked immuno sorbent assay,ELISA)测定TNF-α、hs-CRP、CC16,并进行有关统计学检验.结果表明,与运动对照组相比,不同浓度PM2.5染毒组大鼠运动后,即刻血清TNF-α及hs-CRP水平升高,而CC16水平在染毒组运动后即刻降低,24 h后TNF-α、hs-CRP、CC16基本恢复到对照组的水平且与染毒浓度有剂量相关关系.In this study, the sixty four rats were randomly selected and divided into exercise control group（EC）, recovery for 24 h after exercise（ECR）, 7.5 mg·kg-1（LPE）, 15 mg·kg-1（MPE）, 30 mg·kg-1（HPE）, 7.5 mg·kg-1＋24 h recovery （LPER）, 15 mg·kg-1＋24 h recovery （MPER）, 30 mg·kg-1＋24 h recovery（HPER）, PM2.5 was administered to rats by intratracheal instillation, and then all rats were trained on treadmill with increasing load. Then the rats were killed after exercising immediately and 24 h recovery, and the TNF-α, hs-CRP, CC16 of serum were tested by Enzyme-linked immunosorbent assay （ELISA）. The results show that TNF-α and hs-CRP increased and CC16 decreased after exercising immediately in comparison with the control group. All the indicators fell back to the level of the control group after 24 h recovery and showed correlative dose-response relationship.

关 键 词：细颗粒物 急性运动 肿瘤坏死因子α 超敏C反应蛋白 克拉拉细胞蛋白 

分 类 号：R563.8[医药卫生—呼吸系统] X513[医药卫生—内科学]