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机构地区:[1]西安建筑科技大学环境与市政工程学院,西安710055
出 处:《环境化学》2014年第3期375-380,共6页Environmental Chemistry
基 金:青年科技基金(QN1043)资助
摘 要:采用一次性递增负荷跑台运动对不同浓度PM2.5染毒大鼠进行训练,通过测定含量的变化,研究运动、PM2.5染毒及24 h恢复对大鼠血清肿瘤坏死因子-α(Tumor Necrosis Factor,TNF-α)、超敏C反应蛋白(hypersensitive C-reactive protein,hs-CRP)、克拉拉细胞蛋白(Clara Cell Protein,CC16)的影响机制,选取雄性SD大鼠64只(每组8只),随机分为运动即刻组(EC)、24 h恢复组(ECR)、7.5 mg·kg-1(LPE)、15 mg·kg-1(MPE)、30 mg·kg-1(HPE)、7.5 mg·kg-1+24 h恢复组(LPER)、15 mg·kg-1PM2.5+24 h恢复组(MPER)、30 mg·kg-1+24 h恢复组(HPER).采用气管滴注法PM2.5染毒然后进行一次性递增负荷跑台进行训练,运动即刻组与恢复组分别于训练后即刻及恢复24 h后处死,提取血清后通过酶联免疫法(Enzymelinked immuno sorbent assay,ELISA)测定TNF-α、hs-CRP、CC16,并进行有关统计学检验.结果表明,与运动对照组相比,不同浓度PM2.5染毒组大鼠运动后,即刻血清TNF-α及hs-CRP水平升高,而CC16水平在染毒组运动后即刻降低,24 h后TNF-α、hs-CRP、CC16基本恢复到对照组的水平且与染毒浓度有剂量相关关系.In this study, the sixty four rats were randomly selected and divided into exercise control group(EC), recovery for 24 h after exercise(ECR), 7.5 mg·kg-1(LPE), 15 mg·kg-1(MPE), 30 mg·kg-1(HPE), 7.5 mg·kg-1+24 h recovery (LPER), 15 mg·kg-1+24 h recovery (MPER), 30 mg·kg-1+24 h recovery(HPER), PM2.5 was administered to rats by intratracheal instillation, and then all rats were trained on treadmill with increasing load. Then the rats were killed after exercising immediately and 24 h recovery, and the TNF-α, hs-CRP, CC16 of serum were tested by Enzyme-linked immunosorbent assay (ELISA). The results show that TNF-α and hs-CRP increased and CC16 decreased after exercising immediately in comparison with the control group. All the indicators fell back to the level of the control group after 24 h recovery and showed correlative dose-response relationship.
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