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作 者:孙慧玲[1] 方建光[1] 王清印[1] 燕敬平[1] 李锋[1] 张继红[1] 梁兴明[1] 梁翻鹏[1] 孟瑞平
机构地区:[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]青岛市水产技术推广站,山东青岛266071
出 处:《水产学报》2000年第4期297-301,共5页Journal of Fisheries of China
基 金:国家攀登计划B资助项目!(PDB -6 -3-2 )
摘 要:报道了泥蚶成熟精子在SEM和TEM下的超微结构观察结果。描述了其外部形态和内部构造 ,扫描电镜观察的泥蚶精子呈具有细长鞭毛的蝌蚪状 ,分头部和尾部 ,精子全长约 5 2~ 5 8μm ,头部长约 2 .7~ 2 .9μm。在透射显微镜下 ,根据其结构泥蚶的精子可分为头部、中段和末段。头部顶体明显突出呈倒V字形 ,V字形顶端膨大形成圆球形结构 ;细胞核近似卵圆形 ,是整个精子电子密度最高的部分 ,在细胞核区域内有囊泡存在 ,且在不同的切片样品中囊泡所处的位置完全不同。中段由相互垂直的近、远端中心粒和围绕在其周围的 5个卵圆形线粒体组成 ,中心粒为中空的圆柱形 ;具有卫星体结构。尾部为典型的“9+ 2”结构。Electron microscopy reveals mature spermatozoon of Tegillaca granosa.The observation shows that the spermatozoon of Tegillaca granosa is of flagellate construction in SEM. The body and head length of sperm is 52-58μm and 2.7-2.9μm respectively. The mature spermatozoon consists of head, middle piece and tail in TEM. The acrosome is V shaped in upside down, and the top of V shaped inflate to become a globoid in which there are many tiny particulate material. The unclear is egg shaped with high electron density. The vesicle was found in the unclear region, and it is in different position. Two centrioles and five large globular mitochondras form middle piece of sperm. The centrioles are of cylindrical hollow construction surrounded by five mitochondras bodies and separated from the basal plate of the tail. A satellite body was found at the basal part of the distal centriole. The flagellum is a typical “9+2” microtubular structure limited by the cell membrane.
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