鸣禽白腰文鸟高级发声中枢细胞起源点及其性双态性增殖  

ORIGIN OF LONCHURA STRIATA HVC CELLS AND ITS SEXUALLY DIMORPHIC PROLIFERATION

作　　者：陈琼[1,2] 左明雪[2] 曾少举[2] 

机构地区：[1]广州医学院第三附属医院检验科,广州510150 [2]北京师范大学生命科学学院,北京100875

出　　处：《北京师范大学学报（自然科学版）》2014年第1期58-61,共4页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(31071923;31172082;31372200)

摘　　要：为了更有针对性地研究鸣禽高级发声中枢(HVC)性双态性的形成过程,本文以白腰文鸟(Lonchura striata)为实验对象,首先通过vimentin免疫组织化学分析端脑HVC区域放射胶质细胞纤维的起源与走向,推断出幼鸟孵化后的HVC正背侧VZ区为HVC细胞的主要发源位点;在此基础上,对15d幼鸟经由胸大肌注射3H-胸腺嘧啶以标记端脑新生细胞,通过3 H放射性自显影技术比较雌、雄HVC干细胞的增殖能力,充分证明了HVC细胞发源点的神经干细胞细胞增殖能力具有显著的性别差异.To analyze mechanism of sexually dimorphic development of high vocal center （HVC） in songbird, it was confirmed that ventricular zone （VZ） dorsal to HVC was an important source of HVC cells, and their migratory route was identified by the distribution of radial glial cells in telencephalon in Lonchura striatu with vimentin antibody immunohistology; Male and female Lonchura striatu 15 d post-hatching received intramuscular injection of ^3 H-thymidine, the number of newborn cells in VZ dorsal to HVC was counted by ^3 H- thymidine autoradiography. Such data proved remarkable sex difference in cell proliferation in the origin of HVC cells.

关 键 词：白腰文鸟 高级发声中枢 性双态性 增殖 

分 类 号：Q959.739[生物学—动物学]