吡咯喹啉醌对脂肪肝蛋鸡肝损伤的保护作用机制  

Protective Mechanisms of Dietary Pyrroloquinoline Quinine on Fatty Liver Laying Hens

作　　者：赵芹[1] 张海军[1] 武书庚[1] 岳洪源[1] 王晶[1] 齐广海[1] 孙琳琳 

机构地区：[1]中国农业科学院饲料研究所,农业部饲料生物技术重点开放实验室,北京100081 [2]上海医学生命科学研究中心有限公司,上海200032

出　　处：《动物营养学报》2014年第3期651-658,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(31172212);国家现代农业蛋鸡产业技术体系(CARS-41-K13)

摘　　要：本试验旨在研究吡咯喹啉醌(PQQ)防治蛋鸡脂肪肝综合征的作用机制。选用29周龄海兰褐蛋鸡288只,随机分为4组,每组6个重复,每个重复12只鸡。Ⅰ组为对照组,饲喂基础饲粮(代谢能11.03 MJ/kg,粗蛋白质16.2%);Ⅱ组为病理模型组,饲喂高能低蛋白质饲粮(代谢能12.75 MJ/kg,粗蛋白质13.0%);Ⅲ和Ⅳ组分别在Ⅱ组的高能低蛋白质饲粮基础上添加0.08和0.16 mg/kg的PQQ。试验期4周。结果表明:1)与Ⅰ组相比,Ⅱ组高能低蛋白质饲粮成功诱导了蛋鸡脂肪肝试验模型,表现为肝细胞弥散性脂肪变性,肝脏中甘油三酯、总胆固醇含量显著升高(P<0.05),血浆谷丙转氨酶活性显著升高(P<0.05);2)饲粮PQQ可显著抑制高能低蛋白质饲粮引起的肝脏中甘油三酯和总胆固醇含量及血浆中谷丙转氨酶活性的升高(P<0.05),达到与对照组相当的水平;3)饲粮PQQ可显著抑制高能低蛋白质饲粮引起的肝脏中超氧化物歧化酶活性降低和丙二醛含量升高(P<0.05);4)饲粮PQQ可显著抑制高能低蛋白质饲粮引起的蛋鸡肝脏线粒体相对含量减少(P<0.05),抑制柠檬酸脱氢酶和细胞色素C氧化酶活性降低(P<0.05);5)饲粮PQQ可阻止采食高能低蛋白质饲粮的蛋鸡发生脂肪肝,维持与正常对照组相当的水平,不同剂量PQQ之间未见显著差异(P>0.05)。由此可见,饲粮PQQ可通过改善蛋鸡肝脏线粒体功能、调节脂质代谢和抗氧化功能预防蛋鸡脂肪肝综合征。This experiment was conducted to investigate the protective mechanisms of dietary pyrroloquinoline quinine （ PQQ） on laying hens with fatty liver syndrome. Two hundred and eighty eight Hy-Line brown laying hens aged 29 weeks were randomly divided into 4 groups with 6 replicates per group and 12 hens per replicate. Hens in group Ⅰ （control group） were fed a basal diet （ME 11. 03 MJ/kg; CP 16. 2%）, hens in group Ⅱ（ pathological model control group ） were fed a high-energy low-protein diet （ ME 12 . 75 MJ/kg; CP 13 . 0%） , and hens in groups Ⅲ and Ⅳ were fed the high-energy low-protein diet （ the same as group Ⅱ） supplemented with 0. 08 or 0. 16 mg/kg PQQ, respectively. The experiment lasted for 4 weeks. The results showed as follows：1） compared with the control group, high-energy low-protein diet in groupⅡsuccessfully induced experimental fatty liver model;there were large quantity of fat degeneration in livers from pathological model control; the contents of triglyceride and total cholesterol in liver, and the plasma alanine aminotrans-ferase activity in groupⅡwere significantly increased （ P〈0 . 05 ） . 2 ） Dietary PQQ strikely suppressed the el-evation of contents of triglyceride and total cholesterol in liver, and the plasma alanine aminotransferase activity caused by high-energy low-protein diet （ P〈0 . 05 ） , to the same levels as normal control. 3 ） Dietary PQQ significantly prevented the decrease of superoxide dismutase activity, and the increase of malondialdehyde con-tent induced by high-energy low-protein diet （ P〈0 . 05 ） . 4 ） Dietary PQQ significantly inhibited the decrease of mitochondrial relative content and the activities of citrate synthase and cytochrome C oxidase induced by high-energy low-protein diet （P〈0. 05）. In conclusion, dietary PQQ can prevent the fatty liver syndrome in-duced by high-energy low-protein diets by improving mitochondrial function, and regulating the lipid metabo-lism and anti-oxidative activities in th

关 键 词：吡咯喹啉醌 蛋鸡脂肪肝综合征 高能低蛋白质饲粮 脂质代谢 线粒体 

分 类 号：S831.5[农业科学—畜牧学]