一株奇异变形杆菌对铜绿假单胞菌多细胞行为的抑制作用  

Inhibition of Multicellular Behaviors of Pseudomonas aeruginosa by a Proteus Mirabilis

作　　者：余时琛[1,2] 朱小山[2] 周进[2] 蔡中华[2] 

机构地区：[1]清华大学生命科学学院,北京100084 [2]清华大学深圳研究生院海洋科学与技术学部,广东深圳518055

出　　处：《现代生物医学进展》2014年第6期1001-1004,1008,共5页Progress in Modern Biomedicine

基　　金：国家重点基础发展规划项目(973项目)(2012CB426504)

摘　　要：目的:抗生素耐药性成为了全球性的健康问题。研究发现病原菌的多细胞行为在抗生素的耐药性中起着至关重要的作用(尤其是生物膜),因而通过抑制多细胞行为而控制耐药性成为当务之急。本文以奇异变形杆菌(Proteus mirabilis)为研究对象,考察它的发酵滤液对一种机会致病菌——铜绿假单胞菌(Pseudomonas aeruginosa)多细胞行为的作用,以期得到一株多细胞行为抑制菌:在不影响P.aeruginosa生长的前提下,抑制生物膜形成、EPS产生以及定向丛集运动,解除保护,减缓扩散,为降低P.aeruginosa耐药性,增强抗生素作用效果提供可能。方法:采用结晶紫生物膜测定法、蒽酮-硫酸法、平板检测法,探究P.mirabilis发酵滤液对P.aeruginosa生物膜、胞外多聚物、定向丛集运动和生长的影响。结果:P.mirabilis发酵滤液能显著抑制P.aeruginosa生物膜量,在体积百分比浓度为1%时,抑制率可达60.9%。该菌的发酵滤液还能阻碍P.aeruginosa的定向丛集运动,减弱它的吸附和扩散运动;同时,也减少了P.aeruginosa胞外多聚物的产量,在滤液体积百分比浓度为1%时,抑制率达到45.9%。更重要的是,固体平板实验证明该发酵滤液对P.aeruginosa的生长没有影响。结论:P.mirabilis在不影响病原菌生长的前提下,对病原菌的多细胞行为有一定的控制作用。其发酵滤液中存在着抑制微生物膜、定向丛集运动等的成分,在治疗细菌感染性疾病和降低抗生素耐药性方面有潜在应用价值。Objective： Antibiotic resistance of pathogens has become a global healthcare problem. Many studies have demonstrated that multicellular behaviors are important in the antibiotic resistance of the encased bacteria. Thus it is urgent to control antibiotic resis- tance by inhibiting the multicellular behaviors. The filtrate of cultured Proteus mirabilis isolated from a fi＇eshwater environment, to inhibit the multicellular behaviors （biofilm formation, swarming motility） of an opportunity pathogen-Pseudomonas aemginosa were investigated in this study. We expect to acquire a multicellular behavior inhibitor, which will decrease the biofilm formation, EPS production and swarming motility of P. aemginosa. With the loss of protection and retardation of diffusion, it is possible to control antibiotic resistance and enhance the effect of antibiotic to P. aeruginosa. Methods： According to crystal violet （CV） method, anthrone-H2SO4 assay, plates assay, the effects of filtrate of cultured Proteus mirabilis on biofilm, swarming, extracellular polymeric substances were determined. Re- sults： The cultured P. mirabilis filtrate could effectively inhibit the formation of biofilms of P. aeruginosa with different concentrations （0.1%, 0.5 %, 1%）, and at the concentration of 1%, the inhibition rate reached 60.9%. The filtrate could also suppress the swarming motility of P. aeruginosa, and the dispersal of P. aeruginosa slew down. Moreover, the production of extracellular polymeric substances were significantly decreased, and the highest inhibition rate in this study was 45.9% at the concentration of 1%. Furthermore, the addi- tion of P. mirabilis filtrate did not influence the growth of P. aeruginosa. Conclusion： P. mirabilis can control the multicellular behaviors of P. aeruginosa without inhibiting its growth, and may act as a biofilm inhibitor with potential value in infectious disease treatment and bacterial antibiotic resistance.

关 键 词：生物膜 定向丛集运动 胞外多聚物 奇异变形杆菌 

分 类 号：Q73[生物学—分子生物学] Q936