扁豆过敏原Len c 3蛋白B细胞抗原表位预测及同源建模  

Prediction of the B Cell Antigenic Epitopes and Homology Modeling Study of Lentil Allergen Len c 3

作　　者：郭予和 何颖[1] 邹泽红[1] 刘雪婷[1] 许晨霞[1] 陶爱林[1] 

机构地区：[1]广州市过敏反应与临床免疫重点实验室/呼吸疾病国家重点实验室变态反应研究室/广州医学院第二附属医院,广东广州510260

出　　处：《现代生物医学进展》2014年第6期1017-1021,共5页Progress in Modern Biomedicine

基　　金：国家重大科技专项重大课题(2011ZX08011-005);国家自然科学基金项目(30771240)

摘　　要：目的:通过应用生物信息学软件模拟、分析预测扁豆过敏原Len c 3蛋白的结构、性质及B细胞抗原表位,为探索基于扁豆过敏原Len c 3抗原表位的改造提供依据。方法:从Uniprot蛋白质数据库中得到扁豆过敏原Len c 3的氨基酸序列,通过生物信息学软件Swiss-Model及Swiss-PdbViewer 4.0模拟和分析扁豆过敏原Len c 3蛋白的结构,使用DNAStar软件对其B细胞抗原表位进行模拟、分析预测。结果:扁豆过敏原Len c 3蛋白为疏水性蛋白,在氨基酸残基第7-26位有一跨膜区,Ramachandran图评估扁豆过敏原Len c 3蛋白的三维结构显示其空间构象稳定,Len c 3蛋白潜在的B细胞抗原表位为35-36,48-50,66-71,87-90。结论:本研究通过对扁豆过敏原Len c 3蛋白进行生物信息学分析获得了该过敏原的结构、性质及潜在的B细胞抗原表位,为进一步了解和掌握扁豆过敏原Len c 3蛋白的结构功能以及抗原性改造、单克隆抗体制备、表位疫苗设计等提供重要的线索。Objective： To predict and analyze the structure, biological characteristics and B cell antigenic epitopes of lentil allergen Lenc 3 by using bioinformatics software, so as to provide clues for modifying the antigenic epitopes of lentil allergen Lenc 3. Methods： The amino sequence of lentil allergen Len c 3 was acquired from Uniprot protein database. The structure of lentil allergen L-en c 3 was simulated and analyzed by the bioinformatics software Swiss-Model and Swiss-PdbViewer 4.0, and the B cell antigenic epitopes of lentil allergen Lenc 3 were predicted and analyzed by using DNAStar. Results： Lentil allergen Len c 3 with a transmembrane domain belongs to a hydrophobic protein. The Ramachandran plot showed the spatial conformation of Lentil allergen Len c 3 was stable. The potential B cell antigenic epitopes of Len c 3 were at the region of 35-36, 48-50, 66-71, 87-90. Conclusion： This study predicted and analyzed the structure, biological characteristics and potential B cell antigenic epitopes of lentil allergen Lenc 3, which provided important clues for further understanding and mastering the structure and biological characteristics even antigen modification, monoclonal antibody prepara- tion and epitope vaccine design of Lentil allergen Lenc 3.

关 键 词：扁豆过敏原 Len C 3 结构 抗原表位 

分 类 号：R392.8[医药卫生—免疫学]