姜黄素诱导恶性黑色素瘤细胞凋亡及对AIFM2基因表达的影响  

AIFM2 gene expression and apoptosis induced by curcumin in human malignant melanoma cell line

作　　者：邓辰亮[1] 郑江红[1] 万伟东[1] 杨波[1] 茅广宇[1] 刘斌[1] 杨松林[1] 

机构地区：[1]上海交通大学附属第六人民医院整形外科,上海200233

出　　处：《长春中医药大学学报》2014年第1期9-11,共3页Journal of Changchun University of Chinese Medicine

基　　金：国家自然科学基金(81000837)

摘　　要：目的明确姜黄素诱导恶性黑色素瘤细胞系A375凋亡的作用，探讨姜黄素作用机制。方法A375细胞系经5～20μmol／L姜黄素作用48h后，MTT法测定细胞增殖能力；流式细胞仪检测细胞凋亡；RT-PCR方法检测细胞AIFM2及p53mRNA的表达。结果0、5．0、10．0、20．0μmol／L姜黄素作用A375细胞48h后细胞增殖抑制率分别为1．1％±0．3％、14．9％±5．9％、21．6％±4．7％、41．5％±5．4％；凋亡所占的百分比分别为3．5％±1．8％、17．1％±7．8％、23．8％±6．0％、33．6％±4．4％；AIFM2基因mRNA表达相对比值为（0．6±0．2）、（0．8±0．2）、（1．0±0．4）、（1．2±0．5）；p53血ⅢA表达相对比值分别为（0．9±0．4）、（1．1±0．4）、（1．2．4-0．5）、（1．3±0．4）。各组之间均存在统计学意义（P〈0．05）。伴随姜黄素作用浓度的升高，A375细胞的生长被显著抑制。AIFM2mRNA与p53mRNA表达显著上调。结论姜黄素可能通过上调AIFM2基因的表达促使黑色素瘤细胞凋亡。Objective To explore the mechanism and effect of curcumin on human malignant melanoma cell line A375 apoptosis. Methods A375 cell line were treated by 5 - 20 pmoL/L curcumin. Cell proliferation was measured by MTF. Apoptosis was detected by flow cytometry. RT PCR was used to detect AIFM2 and p53 mRNA expression. Results The cellular prolif- eration inhibition rates of 0, 5.0, 10.0,20.0 μmol/L curcumin on A375 cell were 1.1%± 0.3%, 14.9% ± 5.9%, 21.6% ±4.7%,41.5%±5.4% after 48 hours. The percentages of apoptosis were 3.5% ± 1.8%,17.1% ± 7.8%, 23.8 %± 6.0 %, 33.6 % ± 4.4 %. The relative ratios of AIFM2 gene mRNA were （0.6 ± 0.2）, （0.8 ± 0.2）, （ 1.0 ± 0.4）, （1.2± 0.5）. The relative ratios of p53 mRNA expression were （0.9 ± 0.4）, （ 1.1 ± 0.4）, （ 1.2± 0.5）, （ 1.3 ± 0.4）. There are significant differences among these groups （ P 〈 0.05）. The growth of A375 cell was significantly inhibited with the increase of the concentration of curcumin. AIFM2 mRNA and lO53 mRNA expressions were significantly increased, conclusions Curcumin can increase AIFM2 gene expression to accelerate the apoptosis of melanoma cells.

关 键 词：恶性黑色素瘤 姜黄素 AIFM2基因 凋亡 

分 类 号：R285.5[医药卫生—中药学]