大麦小孢子再生植株气孔保卫细胞长度与倍性的相关性  

Relationship Between the Stomatal Guard Cell Length and Ploidy Level in Barley Microspore Regenerated Plantlets

作　　者：何婷[1] 郭桂梅[1] 陈志伟[1] 杜志钊[1] 高润红[1] 徐红卫[1] 邹磊[1] 卜姝明[1] 黄亦辰[1] 刘成洪[1] 

机构地区：[1]上海市农业科学院生物技术研究所,上海市农业遗传育种重点实验室,上海201106

出　　处：《麦类作物学报》2014年第2期175-180,共6页Journal of Triticeae Crops

基　　金：大麦青稞产业技术体系项目(CARS-05);上海市种业发展项目(沪农科种字(2012)第7号);上海市科委基础研究重点项目(12JC1407800)

摘　　要：为了建立一种快速鉴定大麦小孢子来源植株倍性的方法,对大麦品种＂花30＂不同倍性（种子来源二倍体,小孢子来源单倍体和单倍体加倍系）早期植株的不同叶位、不同叶片部位的气孔保卫细胞长度进行了测定,考察了不同取材部位气孔保卫细胞长度的差异,对种子来源二倍体、小孢子来源单倍体和单倍体加倍材料的气孔保卫细胞长度的分布进行了区分。结果表明,＂花30＂单倍体材料的气孔保卫细胞长度在不同叶位以及不同叶片部位差异较小,而单倍体加倍材料和种子来源二倍体材料受叶片部位影响较大,单倍体和二倍体材料间气孔保卫细胞的长度差异显著,而单倍体加倍材料和种子来源二倍体材料间气孔保卫细胞长度未观察到明显差异。单倍体和单倍体加倍材料气孔保卫细胞的长度值范围分别为26.9-37.7μm和36.7~62.1μm;利用37μm临界值可对大麦小孢子来源的DH群体中的单倍体进行快速区分。In order to establish a rapid method to identify the ploidy levels of plantlets generated from microspores of barley, the length of stomatal guard cell of leaves from seed-germinated diploids, microspore-derived haploids and double haploids （DH） of barley cultivar Hua 30 were measured, and the effects of the position of leaf sampling （the part of leaf, the position of leaf） and the ploidy levels on the length of stomatal guard cell were evaluated, then the distribution of stomatal guard cell length in seed-germinated diploids, microspore-derived haploids and double haploids were determined. The re- suits showed that the leaf and leaf position had little effect on the length of stomatal guard cell of hap- loids, while the leaf and leaf position significantly affected the length of stomatal guard cell of seed- germinated diploids and microspore-derived double haploids. There was significant difference on the length of stomatal guard cell between the leaves of haploids and diploids, while there was no obvious difference observed between seed-germinated diploids and microspore-derived double haploids. The length of stomatal guard cell of microspore-derived haploids and double haploids measured was ranged in 26.9 to 37.7μm and 36.7 to 62.1μm, respectively. The demarcation of 37 μm can be used to identify haploids from the DH population generated from microspores of barley.

关 键 词：大麦 气孔保卫细胞 小孢子 倍性鉴定 

分 类 号：S512.3[农业科学—作物学] S330