Physicochemical bases for protein folding,dynamics,and protein-ligand binding  

作　　者：LI HuiMin XIE YueHui LIU CiQuan LIU ShuQun 

机构地区：[1]School of Mathematics and Computer Science,Yunnan University of Nationalities [2]Laboratory for Conservation and Utilization of Bio-Resources & Key Laboratory for Microbial Resources of Ministry of Education,Yunnan University [3]Teaching and Research Section of Computer,Department of Basic Medical,Kunming Medical University [4]Southwest Biological Diversity Laboratory,Kunming Branch of Chinese Academy of Sciences [5]Key Laboratory for Animal Genetic Diversity and Evolution of High Education in Yunnan Province

出　　处：《Science China(Life Sciences)》2014年第3期287-302,共16页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(31370715,31160181,31360277,30860011);the National Basic Research Program of China(2013CB127500);the Program of Innovation Group of Yunnan Province(2011CI123);Foundation for Key Teacher in Yunnan University(XT412003)

摘　　要：Proteins are essential parts of living organisms and participate in virtually every process within cells. As the genomlc sequences for increasing number of organisms are completed, research into how proteins can perform such a variety of functions has become much more intensive because the value of the genomic sequences relies on the accuracy of understanding the encoded gene products. Although the static three-dimensional structures of many proteins are known, the functions of proteins are ulti- mately governed by their dynamic characteristics, including the folding process, conformational fluctuations, molecular mo- tions, and protein-ligand interactions. In this review, the physicochemical principles underlying these dynamic processes are discussed in depth based on the free energy landscape （FEL） theory. Questions of why and how proteins fold into their native conformational states, why proteins are inherently dynamic, and how their dynamic personalities govern protein functions are answered. This paper will contribute to the understanding of structure-function relationship of proteins in the post-genome era of life science research.Proteins are essential parts of living organisms and participate in virtually every process within cells.As the genomic sequences for increasing number of organisms are completed,research into how proteins can perform such a variety of functions has become much more intensive because the value of the genomic sequences relies on the accuracy of understanding the encoded gene products.Although the static three-dimensional structures of many proteins are known,the functions of proteins are ultimately governed by their dynamic characteristics,including the folding process,conformational fluctuations,molecular motions,and protein-ligand interactions.In this review,the physicochemical principles underlying these dynamic processes are discussed in depth based on the free energy landscape(FEL)theory.Questions of why and how proteins fold into their native conformational states,why proteins are inherently dynamic,and how their dynamic personalities govern protein functions are answered.This paper will contribute to the understanding of structure-function relationship of proteins in the post-genome era of life science research.

关 键 词：free energy landscape entropy-enthalpy non-complementarity RUGGEDNESS driving force thermodynamics kinetics 

分 类 号：Q51[生物学—生物化学] O641.4[理学—物理化学]