慢性丙型肝炎患者外周血中CD4^+Foxp3^+调节性T细胞的变化及其临床意义  

Changes of CD4^+Foxp3^+ regulatory T cells in the peripheral blood of patients with chronic hepatitis C and their clinical significance

作　　者：王萍[1] 胥冰[1] 张丽君[1] 

机构地区：[1]陕西中医学院免疫教研室,咸阳712046

出　　处：《山西医科大学学报》2014年第3期167-169,共3页Journal of Shanxi Medical University

基　　金：陕西中医学院中西医结合免疫研究室青年教师科研启动基金资助项目

摘　　要：目的研究慢性丙型肝炎患者外周血中CD4+Foxp3+调节性T细胞(regulatory T cell,Treg)的变化及其临床意义。方法分别采用流式细胞术、实时定量逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)和酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)分别检测60例慢性丙型肝炎患者及40名健康对照者外周血CD4+Foxp3+Treg细胞百分率、核转录因子foxhead winged-helix box protein 3(Foxp3)mRNA的水平以及血浆转化生长因子-β1(transforming growth factor-β1,TGF-β1)的浓度。结果慢性丙型肝炎患者外周血中CD4+Foxp3+Treg细胞百分率、Foxp3mRNA表达水平及血浆TGF-β1浓度与正常对照组相比均明显升高,差异有统计学意义(P<0.05)。结论慢性丙型肝炎患者外周血中CD4+Foxp3+Treg细胞及TGF-β1水平增高,Treg细胞可能参与了慢性丙型肝炎发病过程。Objective To explore the changes of CD4 ＋ Foxp3 ＋ Treg cells in the peripheral blood of patients with chronic hepatitis C and their clinical significance. Methods The percentage of CD4 ＋ Foxp3 ＋ Treg cells was assessed by flow cytometry and the mRNA level of Foxp3 was detected by real-time quantitative reverse transcription polymerase chain reaction （RT-PCR） in 60 patients with chronic hepatitis C and 40 healthy controls. The plasma levels of TGF-β1 were measured by enzyme linked immunosorbent assay （ELISA）. Results The percentage ofCD4 ＋ Foxp3 ＋ Treg cells,the mRNA expression of Foxp3 and the plasma level of TGF-β1 were significantly higher in patients with chronic hepatitis C than those in healthy controls （P 〈 0. 05）. Conclusion The results suggest that Treg cells may be associated with the development of chronic hepatitis C.

关 键 词：慢性丙型肝炎 调节性细胞 FOXP3 转化生长因子-Β1 

分 类 号：R512.63[医药卫生—内科学]