CdTe-JA偶合物的制备和应用  

Coupling Reaction and Application of Jasmonic Acid and CdTe Quantum Dots

作　　者：高艳红[1] 王玉洁[1] 何桂[1] 俞英[1] 

机构地区：[1]华南师范大学化学与环境学院,广州510006

出　　处：《华南师范大学学报（自然科学版）》2014年第2期50-55,共6页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(20975041;21275056);广东省自然科学基金项目(10251063101000000)

摘　　要：以巯基乙胺(MA)作为修饰剂,在水相条件下合成了稳定的CdTe量子点,优化了合成反应条件;其中用异丙醇做沉淀剂效果好,量子点重新分散到pH值为7.4的Tris-HCl缓冲溶液中,荧光基本保持不变;用N-羟基琥珀酰亚胺(NHS)作偶联剂,量子点与茉莉酸(JA)在pH值为7.4,37℃反应30 min合成了具有识别茉莉酸结合位点的探针.CdTe-JA最大吸收峰与CdTe相比蓝移了约10 nm,抗光漂白性好,并初步对绿豆幼苗根部进行了荧光标记.As a good luminescent material,quantum dot can be a probe for targets in complicated system through the surface modification with recognition function molecule. CdTe quantum dots（QDs） were synthesized in aqueous solution using mercaptoethylamine（MA） as stabilizer. The optimal conditions for synthesis were studied by fluorescence spectroscopy. The purification of CdTe QDs with different solvents and pH values of buffer solution was investigated systematically. The results showed that the purification with twice volume isopropanol was the best.The fluorescence of QDs basically unchanged in pH 7. 4 buffer solution. The jasmonic acid-CdTe conjugates was obtained by coupling CdTe QDs with jasmonic acid（JA） using the Nhydroxysuccinimide（NHS） as a coupling agent. The optimal conditions for coupling reaction are pH 7. 4 and stiring for 30 min at 370C .After the JA molecule linked with CdTe QDs,the maximum absorption peak of jasmonic acid-CdTe conjugates was blue-shifted by 10 nm.The photostability of jasmonic acid-CdTe conjugates was also studied by fluorescence microscope. The jasmonic acid一CdTe conjugates was applied preliminarily in the labeling of root sections of mung bean seedlings.

关 键 词：CDTE 巯基乙胺(MA) 茉莉酸(JA) 偶联 

分 类 号：O655[理学—分析化学]