Goniurosaurus indet．的有效性及中国睑虎属(Squamata：Sauria：Eublepharidae)种间的亲缘关系  

Validity of Goniurosaurus indet. and Genetic Relationships of Goniurosaurus( Squamata: Sauria: Eublepharidae) Species in China

作　　者：张洁[1] 庆宁[1] 易祖盛[2] 王英勇[3] 江晓汕 卢文华 James Lazell 

机构地区：[1]华南师范大学生命科学学院,广东省高等学校生态与环境科学重点实验室,广东省水产健康安全养殖重点实验室,广州510631 [2]广州大学生命科学学院,广州510006 [3]中山大学生命科学学院生物博物馆,广州510275 [4]华南农业大学生命科学学院,亚热带农业生物资源保护与利用国家重点实验室,广州510642 [5]美国罗德岛州生物保护所,美国R102835

出　　处：《华南师范大学学报（自然科学版）》2014年第2期92-98,共7页Journal of South China Normal University(Natural Science Edition)

基　　金：广东省自然科学基金项目(06025054);美国the Falconwood Foundation项目

摘　　要：了解我国睑虎属Goniurosaurus(Squamata:Sauria:Eublepharidae)种间的亲缘关系以及广东新纪录的睑虎属未定种Goniurosaurus indet.的有效性,对睑虎属10个种的线粒体DNA基因片段比较和遗传变异分析.获得12S rRNA(385 bp)和16S rRNA(477 bp)总长度为862 bp的联合序列,共有核苷酸变异位点388个,简约信息位点269个.选取大壁虎Gekko gecko为外类群,采用贝叶斯演绎法(BI)、最大似然法(ML)、最大简约法(MP)、邻接法(NJ)对两个基因联合序列的分析均显示相同结果,即广东睑虎属未定种Goniurosaurus indet.与英德睑虎G.yingdeensis以及凭祥睑虎G.luii与蛛纹睑虎G.araneus两两种间的亲缘关系较近;且分布区相邻的Goniurosaurus indet.与G.yingdeensis之间的遗传分化(遗传距离=0.044-0.046)相似于分类地位已经明确的G.luii与G.araneus种间的遗传差异(遗传距离=0.051).结合形态学研究结果,认为Goniurosaurus indet.和G.yingdeensis之间已发生显著的遗传差异,前者可能为睑虎属新种.同时,系统发育分析表明海南睑虎G.hainanensis与睑虎属其它物种之间的遗传分化较大,系统发育地位最为特殊.系统发育分析进一步表明具4条背部横纹是祖征,具5条背部横纹为衍征.该文首次提出宽阔的河流可能是造成睑虎种群分化和新种形成的重要地理屏障.这一"河流隔离"观点对全球睑虎属物种的亲缘地理分布格局的解释具有重要的理论意义.We studied the genetic differentiation of Goniurosaurus indet. from northwestern Guangdong Province, China, and its phylogenetic relationships with other Chinese species, using partial sequences of the mitochondrial 12S rRNA and 16S rRNA genes of 10 species in total and Gekko gecko as the outgroup. A total sequence of 862 nucleotides of 12S rRNA （385 bp） and 16S rRNA （477 bp） genes were analyzed; 388 variable sites and 123 parsimoniously informative sites were detected in combination of the two genes. Analyses of Bayesian inference （BI）, maximum likelihood （ML）, maximum parsimony （MP）, and neighbor-joining （NJ） all showed similar results： a close phylogenetic relationship between Goniurosaurus indet. and G. yingdeensis （genetic distance = 0.044-0.046）, comparable to that between accepted known species G. araneus and G. luii （genetic distance = 0.051）. Combined with morphological differences, our results support the notion that there is significant genetic differentiation between Goniurosaurus indet. and G. yingdeensis even though the two forms are separated only by a river; thus, Goniurosaurus indet. may be a new species. The phylogenetic analyses also indicated the uniqueness of G. hainanensis with the greatest genetic distance from others, suggesting that the character state of four body bands including nuchal loop is plesiomorphic as compared to that of five body bands. Our study lends support to the known phylogeny of Eublepharidae and provides complementary data for the conservation of Goniurosaurus. We hypothesized for the first time that rivers act as isolatiing barrier for population differentiation and speciation among continental Goniurosaurus species.

关 键 词：睑虎属 线粒体DNA 地理亲缘关系 河流隔离 

分 类 号：Q958.2[生物学—动物学]