子宫内膜异位症PTEN启动子甲基化研究  

Research of promoter hypermethylation of PTEN Gene of Endometriosis

作　　者：任力群 邱丽影 资捷 周晓梅 张银汉[2] 唐丽霞 龙礼华 

机构地区：[1]深圳市福田区妇幼保健院,广东深圳518000 [2]北京大学深圳医院,广东深圳518000

出　　处：《中国热带医学》2014年第1期76-78,共3页China Tropical Medicine

基　　金：深圳市科技计划项目(No.201103159);深圳市福田区公益性科研计划项目(No.201156)

摘　　要：目的检测子宫内膜异位症(EMS)在异位内膜、在位内膜及正常子宫内膜的PTEN启动子甲基化,探讨其与子宫内膜异位症发生发展的关系。方法收集共计45例子宫内膜异位症患者术后子宫内膜异位症在位、异位内膜标本作为病例组及35例正常子宫内膜标本作为对照组,应用巢式甲基化特异性PCR(nMSP)测定PTEN启动子的甲基化,分析PTEN启动子的甲基化与EMS的相关性。结果与对照组比较,病例组PTEN启动子甲基化水平明显提高,甲基化率分别为24.4%(11/45)和0(0/45),差别具有统计学意义(χ2=9.99,P<0.05)。结论 PTEN基因甲基化与子宫内膜异位症的发生发展密切相关。Objective To investigate the relationship between PTEN gene and endometriosis by evaluation of the methylation of the promoter region of PTEN gene in ectopic endometrium and uterine endometrium of endometriosis and normal endometrium. Method Forty-five cases with ectopic endometrium and uterine endometrium of endometriosis and 35 normal endometrium were selected as the observation group and control group. The nested- methylation specific polymerase chain reaction（nMSP） was applied to determine the methylation of the promoter region of PTEN and analyze the relationship of the methylation and endometriosis. Result The methylation in case of observation group was significantly improved compared with the control, and the result was statistically significant（χ2=9.99,P 0.05）. Conclusion The methylation of the promoter region of PTEN gene was closely related with the occurrence and development of endometriosis.

关 键 词：子宫内膜异位症 PTEN 甲基化 

分 类 号：R711.71[医药卫生—妇产科学]