葛根芩连汤对2型糖尿病模型大鼠作用的研究  

Research on the effect of Gegenqinlian Decoction on type 2 diabetic model

作　　者：武志黔[1] 郝改梅[1] 薛晓兴 何洁[1] 吴晏[2] 韩静[2] 王伟[1] 

机构地区：[1]北京中医药大学基础医学院,硕士研究生100102 [2]北京中医药大学基础医学院科研中心,100102

出　　处：《环球中医药》2014年第3期161-167,共7页Global Traditional Chinese Medicine

基　　金：国家科技重大专项"重大新药创制"专项(2009ZX09502-018);北京中医药大学创新团队项目(2011-CXTD-06);北京中医药大学在读研究生资助项目(2013-JYBZZ-XS-027)

摘　　要：目的探索葛根芩连汤单药及联合西格列汀对2型糖尿病模型大鼠血糖值、胰岛素耐量、葡萄糖耐量的作用,从而初步揭示葛根芩连汤治疗2型糖尿病的生物学机制。方法采用高脂饲料喂养合并链脲佐菌素(streptozotocin,STZ)诱导建立2型糖尿病模型大鼠58只,予以无菌水(正常组和模型组)、葛根芩连汤(中药组)、西格列汀(西药组)、葛根芩连汤联合西格列汀(中加西组)给药6周的治疗方案,动态监测各组模型动物体重、空腹血糖(fasting blood glucose,FBG),以及给药前、给药后3周、给药后6周的胰岛素耐量试验和葡萄糖耐量试验。结果给药6周后,与模型组相比,中药组FBG降低(P<0.05),葡萄糖耐量改善(P<0.05);西药组体重降低改善(P<0.05),FBG降低(P<0.05),胰岛素耐量、葡萄糖耐量改善(P<0.05);中加西组体重降低改善(P<0.05),FBG降低明显(P<0.01),胰岛素耐量、葡萄糖耐量均改善明显(P<0.01)。给药各组疗效顺序如下:中加西组>西药组>中药组。结论葛根芩连汤与西格列汀单独使用时,西药组疗效优于中药组,联合使用时其疗效明显优于两药单独使用,表明葛根芩连汤对西格列汀的疗效有一定的辅助作用。Objective To explore the effect of Gegenqinlian Decoction, sitagliptin monotherapy or combination therapy on the blood glucose, insulin tolerance, glucose tolerance of rat model of type 2 diabe- tes, and initially reveal the biological mechanisms of treating type 2 diabetes Gegenqinfian Decoction. Methods 58 high fat diet combined STZ-induced type 2 diabetic model, be given the treatment with water （normal group and model group）, Gegenqinlian Decoction （Chinese medicine group）, sitagliptin （western medicine group） , sitagliptin Gegenqinlian Decoction joint （combination group） , dynamic monitoring model animal body weight, fasting blood glucose （FBG） , and the insulin tolerance tests and glucose tolerance tests of predose, after administration of 3 weeks and 6 weeks in each group. Results After 6 weeks ad- ministration, compared with the model group, Chinese medicine group FBG was lower （ P 〈 0. 05 ） , and has improvement in glucose toleranee （ P 〈 0. 05 ） ; western medicine group improved weight loss （ P 〈 0. 05 ） , FBG was lower （ P 〈 0. 05 ） , and has both insulin resistance and impaired glucose tolerance im- provement （ P 〈 0. 05 ） ; combination group improved weight loss （ P 〈 0. 05 ）, FBG was significantly lower （P 〈 0.01 ）, insulin resistance and impaired glucose tolerance were improved significantly （P 〈 0. 01 ）. The order of efficacy of each group were as follows ： combination group 〉 western medicine group 〉 Chinese medicine group. Conclusion For the monotherapy of Gegenqinlian Decoction or sitagliptin, western medi- cine group was more effective than the traditional Chinese medicine group, while its efficacy when used in combination was significantly better than the two drugs used alone, indicating Gegenqinlian may play a sup- plementary role to the efficacy of sitagliptin.

关 键 词：葛根芩连汤 西格列汀 2型糖尿病 胰岛素耐量 葡萄糖耐量 

分 类 号：R255.4[医药卫生—中医内科学]