白藜芦醇对酒精性氧化损伤修复作用研究  

Reparative Function of Resveratrol in Ethanol-induced Oxidative Damage

作　　者：童春义[1,2] 李芳 卜晓英[3] 蒋斌[1,2] 张志美[1,2] 唐凤霞 刘选明[1,2] 

机构地区：[1]湖南大学生物学院,湖南长沙410082 [2]植物功能基因组学与发育调控湖南省重点实验室(湖南大学),湖南长沙410082 [3]吉首大学林产化工工程湖南省重点实验室,湖南张家界427000

出　　处：《湖南大学学报（自然科学版）》2014年第3期79-82,共4页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金资助项目(31201074);湖南省自然科学基金资助项目(11JJ4073;11JJ6018)

摘　　要：以4.8％（（6）乙醇）酒精处理HeLa细胞12 h建立酒精氧化损伤模型,通过MTT实验、流式细胞术、生化分析实验、定量PCR等手段和技术研究了白藜芦醇对酒精氧化损伤细胞的修复作用.结果显示：白藜芦醇处理酒精氧化损伤模型细胞24 h,细胞形态得到恢复,存活率提高,细胞内MDA含量由3.56 nmol/mg下降到1.42 nmol/mg,T-SOD活性从61.62 U/mg增强到87.78 U/mg,氧化损伤相关基因OGG1和SIRT1表达量增加了2～4倍.实验表明,白藜芦醇作用细胞后使其减少了MDA含量、增强T-SOD活性并通过上调OG-G1和SIRT1的表达减轻和修复酒精对细胞造成的氧化损伤.HeLa cells treated with ethanol Ior to study the intervention of oxidative damage cells with resveratrol （RES）. Varied technologies were used for analysis, such as MTT assay, flow cytometry, biochemical analysis and real time PCR et al. After the addition of RES for 24 h, the morphology of most cells was recovered, the survival rate was improved, the content of MDA was decreased from 3.56nmol/mgprot to 1.42nmol/mgprot, the T-SOD activity was in creased from 61.62U/mgprot to 87.78U/mgprot and the expression of OC1 and SIRT1 was up-regulated 2-4 times. The results indicated that RES could repair ethanol-induced oxidative damage probably by re ducing MDA, improving T-SOD activity and up-regulating expression of OCGI and SIRT1. This study can provide a theoretical basis for the application of RES.

关 键 词：白黎芦醇 酒精 氧化 损伤 修复 

分 类 号：O629.9[理学—有机化学]