iNOS基因rs10459953/rs1060822多态性与低氧训练效果的关联性研究  

Association between polymorphisms rs10459953 /rs1060822 gene and variation in response to hypoxia training

作　　者：毛颖颖[1] 汪洪波[1] 刘海平[1,2] 

机构地区：[1]温州医科大学体育科学学院,温州浙江325035 [2]温州医科大学低氧医学研究所,温州浙江325035

出　　处：《山东体育学院学报》2014年第1期85-89,共5页Journal of Shandong Sport University

基　　金：国家自然科学基金资助项目(30470834);浙江省教育厅2013年度科研课题资助项目

摘　　要：目的:选取低氧反应基因——诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)基因序列上rs10459953、rs1060822单核苷酸多态性(single nucleotide polymorphism,SNP)位点,研究其与低氧训练后生理表型指标关联性。方法:采用关联研究方法(AssociationStudy),聚合酶链反应-限制片段长度多态性(PCR-RFLP)实验解析技术,选取35名健康受试者在模拟海拔2500 m高度进行4周高住-高练-低训(living high-exercise high-training low,Hi-Hi-Lo)。研究iNOS基因SNP rs10459953和SNPrs1060822与低氧训练后最大摄氧量(maximal oxygen uptake,VO2max)、血红蛋白(hemoglobin,Hb)、红细胞数(red blood cell,RBC)等生理表型指标关联性。结果:1)4周低氧训练后,SNP rs10459953不同基因型之间VO2max、Hb、RBC变化量均无显著性差异。2)4周低氧训练后,SNP rs1060822不同基因型间Hb、RBC变化量有统计学意义(P<0.05),其中CT基因型受试者Hb、RBC显著增加(P<0.05),且在低氧环境下定量负荷实验中,SNP rs1060822的CT基因型受试者低氧训练后期SpO2动态曲线显著高于初期(P<0.05)。结论:iNOS基因SNP rs1060822与低氧训练后血象指标存在一定关联性,CT基因型表现出一定优势。Objective： In this study, we selected two single nucleotide polymorphisms （SNP）, rs10459953 and rs1060822, in the hypoxia response gene, inducible nitric oxide synthase（iNOS） , to investigate association between these polymorphisms of iNOS gene and physiological phenotype parameters response to hypoxia training. Methods ：35 healthy subjects completed a g weeks＂living high - exercise high - training low＂ in a simulated ahitude of 2500m height, and using association - study of polymerase chain reaction- restriction fragment length polymorphism（ PCR- RFLP） for genotype analysis. To investigate association between SNP rs10459953 and rs1060822 of iNOS gene and physi- ological phenotype parameters including maximal oxygen uptake （VO2max）, hemoglobin （Hb）, red blood cell （ RBC ） and other parameters after hypoxia training. Results ： （ 1 ） After the 4 weeks hypoxia ＇ training,there was no significant difference in VOEmax, Hb or RBC between different genotypes of SNP rs10459953. （2） After the 4 weeks hypoxia training, carriers with CT genotype of SNP rs1060822 showed significantly higher increase in Hb and RBC （ P 〈 0.05 ）. There were significant differences in carriers with CT genetype of SNP rs1060822 in SpO2 during period of exercising before and after 4 weeks hypoxia training （ P 〈 0.05 ） , and SpO2 curve was higher after hypoxia training. Conclusion ：These findings suggested that SNP rM060822 of iNOS gene was possibly associated with hemogram parameters after hypoxia training, and CT genotype showed a certain advantage.

关 键 词：诱导型一氧化氮合酶 单核苷酸多态性 低氧训练 

分 类 号：G804.7[文化科学—运动人体科学]