微量淋巴细胞毒技术与核酸扩增荧光技术检测HLA-B27的对比  

Micro-lymphocytotoxicity test and PCR-dye assay in detection of HLA-B27:A comparative study

作　　者：丛贤滋[1] 李晓峰[1] 黄烽[1] 

机构地区：[1]解放军总医院风湿科实验室,北京100853

出　　处：《解放军医学院学报》2014年第3期280-282,共3页Academic Journal of Chinese PLA Medical School

摘　　要：目的通过比较核酸扩增荧光技术(PCR-染料法)和微量淋巴细胞毒技术在检测HLA-B27中的符合率,分析两种方法的优势与不足,探讨核酸扩增荧光技术(PCR-染料法)在HLA-B27检测中的临床应用价值。方法随机选取197例来我科就诊的疑似强直性脊柱炎(ankylosing spondylitis,AS)患者的样本,分别使用微量淋巴细胞毒法和PCR-染料法检测。并对两种方法检测结果不符的样本进行测序复核。结果 197例样本中,微量淋巴细胞毒法检出HLA-B27阳性150例,阴性47例;PCR-染料法检出阳性135例,阴性62例;两种方法检测结果有统计学差异(P<0.01)。15例差异样本,两种方法与测序法相比较的符合率,微量淋巴细胞毒法为13.3%,PCR-染料法为86.7%。结论核酸扩增荧光技术(PCR-染料法)和微量淋巴细胞毒法比较,前者具有更高的临床准确性。在样本处理上PCR-染料法也更具优势,操作简单方便,有很强的临床应用价值。Objective To study the application of PCR-dye assay and micro-lymphocytotoxicity test in detecting HLA-B27 by analyzing their advantages and disadvantages. Methods HLA-B27 was detected in 197 patients with ankylosing spongylitis （AS） by PCR-dye assay and micro-lymphocytotoxicity test, respectively. The inconsistent findings in PCR-dye assay and micro-lymphocytotoxicity test were tested by sequencing. Results Micro-lymphocytotoxicity test showed positive and negative HLA-B27 in 150 and 47 out of the 197 patients. PCR-dye assay displayed positive and negative HLA-B27 in 135 and 62 out of the 197 patients （P〈0.01）. The HLA-B27 detection rate was significantly higher by micro-lymphocytotoxicity test than by PCR-dye assay in 15 patients with different findings （86.7%vs 13.3%, P〈0.05）. Conclusion The accuracy of PCR-dye assay is better than that of micro-lymphocytotoxicity test in detecting HLA-B27 and is advantageous over micro-lymphocytotoxicity test in sample processing and easier to perform, thus having a bright future in clinical application.

关 键 词：HLA-B27 强直性脊柱炎 微量淋巴细胞毒试验法 核酸扩增(荧光PCR-染料法) 

分 类 号：R593.23[医药卫生—内科学]