黄连解毒汤对高脂血症小鼠血脂及炎症因子的影响  

Effect of Huanglian Jiedu Decoction on Blood Lipid and Inflammatory Factors in Hyperlipidemic Mice

作　　者：邝枣园[1] 宋梦微[2] 马嫚[2] 张韧[1] 李岩[1] 

机构地区：[1]广州中医药大学基础医学院微生物与寄生虫学教研室,广东广州510006 [2]广州中医药大学基础医学院研究生部,广东广州510006

出　　处：《广州中医药大学学报》2014年第2期268-271,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省科技计划项目(编号:2012B031800230);广东省普通高校重点实验室中医病机与治法研究实验室开放课题(编号:2011001214);广州中医药大学基础医学院综合基金研究生类研究项目(编号:BMC043)

摘　　要：【目的】探讨黄连解毒汤对高脂血症小鼠血脂及血清炎症因子水平的调控作用。【方法】选用48只雄性C57BL/6小鼠,随机平均分为6组,即常规饲料组、高脂饲料组、常规饲料加抗生素组、高脂饲料加抗生素组、中药低剂量组(剂量为3.9g·kg-1·d-1)、中药高剂量组(剂量为7.8 g·kg-1·d-1)。常规饲料组和高脂饲料组分别进食常规饲料和高脂饲料8周;中药高、低剂量组进食高脂饲料6周后分别给予高、低剂量中药,连续灌胃2周;抗生素组分别进食常规饲料和高脂饲料6周后给予抗生素自由饮用2周。8周末处死各组小鼠,测定各组小鼠体质量、血脂(TC)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、内毒素水平。【结果】黄连解毒汤低剂量组能显著降低TNF-α、IL-6、内毒素的水平(均P<0.01);但对血清总胆固醇无明显改善作用。【结论】黄连解毒汤改善高脂血症炎症状态的作用与其能降低血中炎症因子水平有关。Objective To observe the regulatory effect of Huanglian Jiedu Decoction （HJD） on lipid metabolism and inflammatory factors in hyperlipidemic mice. Methods Forty-eight male C57BL/6 mice were randomly divided into 6 groups, namely conventional forage group, high-fat forage group, conventional forage plus antibiotics group, high-fat forage plus antibiotics group, low- and high-dose Chinese medicine groups. Mice in conventional forage group and high-fat forage group were fed with the corresponding forage for 8 weeks. Mice in low- and high-dose Chinese medicine groups were given gastric garage of 3.9 and 7.8 g.kg-1.d-1 of Huanglian Jiedu Decoction respectively for 2 weeks after fed with high-fat forage for 6 weeks. Mice in both antibiotics groups received drink water containing antibiotics freely for 2 weeks after fed with conventional forage or high-fat forage for 6 weeks. At the end of experiment, mice in all of the groups were executed, and body mass, total cholesterol （TC）, tumor necrosis factor （TNF） -α, interleukin 6 （IL-6）, and endotoxin were determined. Results Low-dose Huanglian Jiedu Decoction could significantly decrease the contents of TNF-α, IL-6 and endotoxin, but had no effect on serum TC. Conclusion The therapeutic mechanism of Huanglian Jiedu Decoction for inflammatory state of hvDerliDidemic mice is probably related with the decrease of serum inflammatorv factors.

关 键 词：黄连解毒汤 药理学 高脂血症 中药疗法 血脂 炎症因子 血液 疾病模型 动物 小鼠 

分 类 号：R285.5[医药卫生—中药学]