几种基于pCAMBIA系列多用途新型植物表达载体的改建及优化  

Reconstruction and Optimization of pCAMBIA-Based Plant Expression Vectors for Multifunctional Application

作　　者：李敬涛[1] 孙新华[1] 余刚[1] 贾承国[1] 杜茜[2] 李启云[2] 潘洪玉[1] 

机构地区：[1]吉林大学植物科学学院,长春130062 [2]吉林省农业科学院,长春130124

出　　处：《吉林大学学报（理学版）》2014年第2期371-375,共5页Journal of Jilin University:Science Edition

基　　金：国家科技支撑计划项目(批准号:2012BAD19B04);农业部抗病虫转基因大豆新品种培育项目(批准号:2013ZX08004004)

摘　　要：以pCHF3,pCAMBIA1301,pCAMBIA3300和pCAMBIA3301载体为骨架,构建CaMV 35S和rd29A启动子驱动多克隆位点(MCS:SacⅠ,KpnⅠ,SmaⅠ,BamHⅠ,XbaⅠ,SalⅠ,PstⅠ)的通用型重组植物双元表达载体,得到两种启动子驱动下MCS与eGFP融合的应用型亚细胞定位双元表达载体,并建立了对大量候选基因进行高通量筛选和功能验证的通用型表达载体系统.To supply the required large amounts of functional recombinant plant expression vectors,a series of general plant binary expression vectors with the CaMV35S and the rd 29A promoter mediating multiple cloning sites （MCS：SacⅠ,KpnⅠ,SmaⅠ,BamⅠ,XbaⅠ,SalⅠ,P stⅠ）were constructed successfully, on the basis of pCHF3, pCAMBIA1301, pCAMBIA3300 and pCAMBIA3301 vectors,and a set of applicative binary expression vectors utilized for subcellular localization by the fusion of MCS and eGFP with these two promoters were also constructed. Subsequently, the universal high-throughput and expense-saving gene expression system was established for the scan and functional characterization of a large number of candidate genes.

关 键 词：植物双元表达载体 CaMV 35S RD29A 多克隆位点 增强型绿色荧光蛋白 

分 类 号：Q782[生物学—分子生物学]