肠球菌的耐药特征及高水平庆大霉素耐药基因流行分布  

The antimicrobial resistance pattern of Enterococcus and its high-level gentamicin resistance genes distribution

作　　者：王蓉[1] 吴农欣[1] 陈端[1] 单斌[1] 

机构地区：[1]襄阳市中心医院,湖北襄阳441021

出　　处：《国际检验医学杂志》2014年第6期693-694,共2页International Journal of Laboratory Medicine

摘　　要：目的了解临床分离的肠球菌的耐药特征以及高水平庆大霉素耐药肠球菌(HLGRE)的耐药基因流行分布。方法采用Vitek-2全自动微生物鉴定和药敏分析系统对肠球菌进行耐药情况分析,运用聚合酶链反应(PCR)方法检测HLGRE的耐药基因,并对部分PCR扩增产物进行DNA测序。结果 89株肠球菌中,对高水平庆大霉素耐药的屎肠球菌和粪肠球菌分别占50%和25.6%,对82株肠球菌进行高水平庆大霉素耐药基因的检测显示:aac(6')-Ie-aph(2")-Ia基因是HLGRE的唯一耐药基因,未检测到aph(2")-Ib和aph(2")-Id基因。结论准确及时发现肠球菌的耐药性和应用分子生物学技术检测高水平庆大霉素耐药基因可以为临床医师治疗肠球菌感染提供可靠依据。Objective To investigate the antimicrobial resistance pattern of 89 enterococcal isolates and determine the prevalence of the high-level gentamicin resistance genes. Methods Vitek-2 automatic microbial analysis system was used to identify the organ-isms and study the antimicrobial resistance. Polymerase chain reaction （PCR） was applied for detecting high-level gentamicin resist-ance genes. Some suspected PCR products were confirmed by DNA sequencing. Results Totally 89 clinical strains of Enterococcus were isolated. High-level gentamicin resistance was found in 25.6% of Enterococcus faecalis and 50 % of Enterococcus faecium re- spectively. 82 strains of Enterococcus were tested for the presence of high-level gentamicin resistance genes. Aac（6″）-le-aph（2″）-Ia was the only high-level gentamicin resistance gene detected, aph （2″）-Ib and aph （2″）-Id were not detected. Conclusion Under-standing the resistance pattern of high-leveL aminoglycoside resistance and applying molecular biological techniques to detect high-level gentamicin resistance genes in Enterococcus infection are important for rational treatment of infections caused by Enterococcus.

关 键 词：肠球菌属 庆大霉素类 基因 耐药 

分 类 号：R446.5[医药卫生—诊断学]