儿童支气管肺炎患者病程各阶段细胞免疫和体液免疫的改变及临床意义  

Changes and clinical significance of cell immunity and humoral immunity in various stages of child bronchial pneumonia

作　　者：孙林春[1] 张利[2] 张兰芳[1] 胡正[1] 

机构地区：[1]南京医科大学附属南京儿童医院儿科研究所,江苏南京210008 [2]江苏省中西医结合医院检验科,江苏南京210009

出　　处：《国际检验医学杂志》2014年第6期695-696,702,共3页International Journal of Laboratory Medicine

摘　　要：目的检测145例儿童支气管肺炎患者细胞免疫和体液免疫功能在病程各阶段中的改变,探讨其临床意义。方法采用流式细胞术对145例支气管肺炎患儿和50例健康儿童的外周血CD3+、CD3+CD4+和CD3+CD8+细胞进行检测。用免疫散射浊度法对145例支气管肺炎患儿和50例健康儿童的血清免疫球蛋白G(IgG)、免疫球蛋白A(IgA)和免疫球蛋白M(IgM)进行检测。结果支气管肺炎患儿发病时外周血CD3+、CD3+CD4+、CD3+CD8+细胞的相对计数和CD4+/CD8+比值与对照组比较差异有统计学意义(P<0.05);血清IgG和IgA均比对照组降低(P<0.05)。接受3d对症治疗后患儿外周血CD3+、CD3+CD4+细胞的相对计数和CD4+/CD8+比值与对照组比较差异有统计学意义(P<0.05);血清中IgG和IgA比对照组降低(P<0.05)。无临床症状,遵医嘱结束治疗后患儿外周血CD3+、CD3+CD4+、CD3+CD8+细胞的相对计数和CD4+/CD8+比值与对照组比较差异无统计学意义(P>0.05);血清中IgG、IgA和IgM与对照组比较差异无统计学意义(P>0.05)。结论支气管肺炎患儿同时存在细胞免疫和体液免疫功能紊乱,免疫功能处于抑制状态,合理有效的治疗有助于恢复正常的免疫功能。对患儿免疫功能检测可帮助判断病情,指导治疗和评估预后。Objective To assay the changes of the cell immunity and humoral immunity function in various stages of child bron-chial pneumonia and to investigate their clinical significance. Methods The flow cytometry （FCM） was used to detect the peripher-al blood CD3^＋ （T cells） ,CD3^＋ CD4^＋ （Th cells） and CD3^＋ CD8^＋ （Ts cells） cells in 145 children patients with bronchial pneumonia and 50 healthy children. The immunoturhidimetry was used to assay serum immunoglobulins IgG,IgA and IgM. Results The rela-tive counts of CD3^＋ ,CD3^＋ CD4^＋ ,CD3^＋ CD8^＋ cells and CD4^＋/CD8^＋ ratio after onset in the children patients had differences com-pared with the control（P〈0.05） ;serum IgG and IgA were decreased compared with the control group（P〈0.05）. After 3 d symp-tomatic treatment,the relative counts of CD3^＋, CD3^＋ CD4^＋, CD34 CD8^＋ cells and CD4^＋ /CD8^＋ ratio had statistical differences compared with the control group （P〈0.05） ;serum IgG and IgA were decreased compared with the control group（P〈0.05）. After the end of treatment following the medical orders without symptoms, the patients＇ CD3^＋ , CD3^＋ CD4^＋ , CD3^＋ CD8^＋ cells, CD4^＋/ CD8^＋ ratio and IgG,IgA and IgM in serum all had no differences compared with the control group（P〈0.05）. Conclusion The dysfunction of both cellular immune and humoral immune function exist in children patients with bronchial pneumonia and their im-mune function is in depressed state. The rational and effective treatment are conducive to the recovery of normal immune function. Detecting the immune function can help to judge the disease condition and guide the treatment and evaluate the prognosis.

关 键 词：支气管肺炎 淋巴细胞亚群 免疫球蛋白类 

分 类 号：R725.6[医药卫生—儿科]