两种方法检测血清抗CCP抗体在RA诊断中的比较  

Comparison of Two Methods for the Detection of Serum Anti CCP Antibody in the Diagnosis of RA

作　　者：刘国瑞[1] 常林[2] 郑田[1] 严孝岭[1] 王艾丽[1] 虞伟[1] 

机构地区：[1]南京军区南京总医院全军临床检验医学研究所临床中心实验科,南京210002 [2]南京市儿童医院检验科,南京210008

出　　处：《现代检验医学杂志》2014年第1期83-84,87,共3页Journal of Modern Laboratory Medicine

摘　　要：目的比较ELISA法和化学发光法检测血清抗环瓜氨酸肽（CCP）抗体对RA患者的诊断价值和临床适用性。方法收集南京军区南京总医院住院患者和体检健康者血清151例，同时使用酶联免疫吸附试验（ELISA）和化学发光法检测待测样本中抗CCP抗体含量，评价两种方法检测结果的相关性及对临床诊断的有效性。结果两方法检测RA组血清抗CCP抗体含量均显著高于非RA组（P均〈0．01）；ELISA和化学发光法的敏感度、特异度和诊断一致性分别为78．6％，89．5％，85．4％和76．8％，94．7％，88．1％；两方法间比较r＝0．885，P＝0．07〉0．05，方法间无显著性差异；ELISA和化学发光法R0c曲线下面积分别是0．97和0．933。结论血清抗CCP抗体检测对RA患者具有较高的诊断价值，ELISA和化学发光法检测抗CCP抗体符合率良好，方法学之间无统计学差异。Objective Comparing the diagnostic value and clinical applicability of ELISA kit and chemiluminescence method for detecting anti-CCP antibodies of patients with RA. Methods Collected 15t serum samples from Nanjing General Hospi- tal of Nanjing Command, including hospitalized patients and healthy subjects. Using enzyme-linked immunosorbent assay （ELISA） and chemiluminescence to detect anti-CCP antibody levels in the sample, evaluate the two methods and the effec- tiveness of the clinical diagnosis. Results Both methods detected the RA group, serum anti-CCP antibody levels were signifi- cantly higher than non-RA group （P〈0.01）. Sensitivity, specificity and diagnostic consistency of the ELISA and chemical luminescence method were 78.6%,89.5%,85.4% and 76.8%,94.7%,88. 1%. Between the two methods,r=0. 885,P= 0.07%0.05, there were no significant difference. Two methods group the area under the ROC curve was 〉0.9. Conclusion Serum anti-CCP antibody test for RA patients with high diagnostic value of ELISA and chemical luminescence method to de- tect anti-CCP antibody coincidence rate,no statistical significance between the methodologies.

关 键 词：类风湿关节炎 酶联免疫吸附试验(ELISA) 化学发光 抗环瓜氨酸肽抗体 

分 类 号：R593.22[医药卫生—内科学] R392.11[医药卫生—临床医学]