花生四烯酸和二磷酸腺苷浓度对血小板聚集的影响  

Effect of Arachidonic Acid and Adenosine Diphosphate Concentration on Platelet Aggregation

作　　者：陈兴明[1] 吴樵[2] 李祖兰 黄永娣 冯熹文 王红霞 张艳萍 万璐 郭研 丛玉隆[5] 

机构地区：[1]解放军306医院检验科,北京100101 [2]解放军306医院神经内科,北京100101 [3]总装后勤亚运村门诊部,北京100101 [4]总装后勤部辰芳园卫生所,北京100101 [5]解放军总医院临检科,北京100853

出　　处：《现代检验医学杂志》2014年第1期117-118,121,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨花生四烯酸（AA）和二磷酸腺苷（ADP）浓度对血小板聚集的影响。方法随机收集47例门诊体检健康志愿者静脉血标本，枸橼酸钠抗凝，离心后分离富血小板血浆（PRP）和贫血小板血浆（PPP），AA（0．4，0．5，0．7，0．8，1．0，1．5和2．0mmol／L）或ADP（2．5，5，7．5，10，15，20，40和100μmol／L）各浓度上机，采用血浆比浊法测血小板聚集率。结果在诱导剂AA浓度在0．4～2．0mmol／L时，血小板聚集率为（53．9±29．5）％～（64．0±14．5）％（F=5．19，P〈0．05）；在诱导剂ADP浓度在2．5～100／μmol／L时，血小板聚集率为（51．5±8．7）％～（74．9±7．1）0A（F=78．44，P〈0．05）。AA诱导血小板聚集在AA0．7mmol／L时血小板聚集率最高，随AA浓度进一步增高，聚集率有降低的趋势；ADP诱导血小板聚集率随ADP浓度逐渐增高而增高，ADP浓度与血小板聚集率呈线性相关（P〈0．05，r=0．7649）。结论诱导剂浓度对血小板聚集的影响明显，在PRP血小板浓度250×10-9／L左右时，诱导剂AA0．7mmol／L和ADP10μmol／L为诱导血小板聚集的适宜浓度。Objective To detect the effect of arachidonic acid （AA） and adenosine diphosphate （ADP） concentration on test results of platelet aggregation. Methods Blood samples of outpatient medical volunteers （n=47） were collected and antico- agulaged by sodium citrate,then platelet-rich plasma （PRP） and platelet-poor plasma （PPP） was obtained by centrifuging. The rate of platelet aggregation were detected following standard protocols by plasma turbidimetry with inducer of AA （0.4,0.5,0.7,0.8,1.0,1.5 and 2.0 mool/L） or ADP （2.5,5,7.5,10,15,20,40 and 100 μmol/L）. Results When AA and ADP concentration was in the range of 0.4-2.0 mmol/L and 2.5-100 μmol/L respectively,the rates induced by AA and ADP were （53.9±29.5）%（64.0±14.5）% （F=5.19,P〈0.05） and （51.5± 8.7）%（74.9±7.1）% （F=78.44, P〈0.05） respectively. The rate of AA became maximal at the AA concentration of 0.7 mmol/L and then tended to decrease with a higher AA, the rate of ADP gradually increased with a higher concentration of ADP and showed a linear correlation （r =0. 764 9,P〈0.05）. Conclusion The AA and ADP concentration of 0.7 mmol/L and 10 μmol/L seems to be the optimal concentration at the platelet concentration of 250× 109/L.

关 键 词：血小板 血小板聚集 富含血小板血浆 花生四烯酸 二磷酸腺苷 比浊法 

分 类 号：R446.111[医药卫生—诊断学]