荧光显微镜研究极端pH值诱导磷脂支撑膜的侧向再组织  

Extreme pH-induced lateral reorganization of supported lipid bilayer by fluorescence microscope

作　　者：盛洁[1,2] 张国梁[2] 李玉强[3] 朱涛[1] 蒋中英[1,2] 

机构地区：[1]南京大学物理学院,南京210093 [2]伊犁师范学院电子与信息工程学院,伊宁835000 [3]伊犁师范学院物理科学与技术学院,伊宁835000

出　　处：《物理学报》2014年第6期349-356,共8页Acta Physica Sinica

基　　金：国家重点基础研究发展计划(批准号:2012CB821500);国家自然科学基金(批准号:21264016;21364016)资助的课题~~

摘　　要：利用荧光显微镜研究了极端pH值诱导支撑磷脂双层膜的侧向再组织.结果表明,在强酸/强碱性溶液中,流动性较好的二油酰磷脂酰胆碱支撑膜出现破裂、分离、出芽或生出微管等与细胞内吞和外排相似的现象.基于极性分子与H+/H3O+或OH-的相互作用,以电中性的磷脂首基为核吸附溶液中的H+/H3O+或OH-.当磷脂膜上下叶吸附的电荷量不同时,引起两叶有效面积差,即磷脂膜曲率不对称,从而诱发磷脂膜出现各种结构和动力学的响应.本研究有助于理解极端环境对生物膜的影响,为研究生物膜的形变过程提供了参考.The extreme pH-induced lateral reorganization of supported lipid bilayer membranes are studied by fluorescence microscopy. The results show that the fluid dioleoyl-phosphatidylcholine bilayers in extreme acidic or basic solution presents a similar phenomenon to endocytosis and exocytosis, such as rupture, detachment, budding, formation of microtubules etc. In the view of the interaction of polar molecules with H^＋/H3O^＋ or OH ions, we conclude that the zwitterionic phospholipid headgroup as core adsorbs the H^＋/H3O^＋ or OH ions in electrolyte solution. The asymmetric charge adsorption quantity of the lipid headgroups leads to the effective area discrepancy between the outer and inner leaflets of lipid bilayers. The asymmetric membrane curvatures induce a variety of structures and dynamic responses. The present study helps explain lipid membranes reorganization under extreme pH conditions and provides some guidelines for deformation process of lipid membranes.

关 键 词：支撑磷脂双层膜 PH值 膜曲率 侧向再组织 

分 类 号：Q73[生物学—分子生物学]