草莓ABA的快速提取方法及超高效液相色谱分析  

Rapid Method for the Extraction of Abscisic Acid from Strawberry and Analysis by Ultra-high Performance Liquid Chromatography

作　　者：马宏棋[1] 陈敏氡[1] 朱海生[1] 温庆放[1] 

机构地区：[1]福建省农业科学院作物研究所,福建省农业科学院蔬菜研究中心,福建省蔬菜工程技术研究中心,福州350013

出　　处：《园艺学报》2014年第3期577-584,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31101534);福建省农业科学院科技创新团队重点科研项目(CXTD2011-20)

摘　　要：采用甲醇︰乙酸乙酯︰甲酸=50︰50︰1的混合液作为提取溶剂,可快速从草莓中提取ABA,整个提取过程可在0.5 h内完成,大大提高了ABA提取效率。建立了一套适合ABA分析的超高效液相色谱分析体系,其流动相为甲醇︰乙腈︰0.1%甲酸=20︰10︰70,流速0.3 mL·min-1,检测波长265 nm,柱温30℃,进样10μL。利用建立的快速提取法及超高效液相色谱分析方法,测定了不同发育阶段草莓果实(青果、白果、粉红果和红果)的ABA含量,发现其呈上升趋势,至红果期ABA含量达到最高。This study was carried out to look for an optimized method for extraction of endogenous abscisic acid （ABA） from strawberry and for ultra performance liquid chromatography （UPLC） analysis. A rapid extracting method that using Methanol-ethyl acetate-formic acid （50： 50： 1, v/v） as extracting liquid was found out, which can extract ABA from strawberry in half an hour. An efficient UPLC analysis method for ABA was also established, Chromatographic separation was carried out by reversed Phase ACQUITY UPLC BEH C18 column and with Methanol ： acetonitrile ： 0.1% formic acid （20 ： 10 ： 70） isocratic elution program, at column temperature of 30 ℃, injection volume of 10 μL, flowrate of 0.3 mL· min^-1, and wavelength at 265 nm. Applying the extracting and UPLC analysis method, we evaluated the content of ABA in strawberry fruit at different developments and ripening stages. The result showed that with developing and ripening, content of ABA in strawberry was increasing, and reached the highest level at thered ripening stage.

关 键 词：草莓 脱落酸 超高效液相色谱 提取 

分 类 号：S668.4[农业科学—果树学]