紫草素对白介素-22介导永生化角质形成细胞株增殖、迁移及分化的影响  

Effects of shikonin on the proliferation,migration and differentiation of HaCaT cells mediated by IL-22

作　　者：朱晓芳 林向飞 刘国华 陈菲菲 刘燕茹 孟松树[2] 卜平[3] 

机构地区：[1]扬州大学临床医学院皮肤科,江苏扬州225001 [2]扬州大学生命科学与技术学院,江苏扬州225009 [3]扬州大学医学院中西医结合临床系,江苏扬州225001

出　　处：《临床皮肤科杂志》2014年第4期215-218,共4页Journal of Clinical Dermatology

摘　　要：目的:研究紫草素对白介素(IL)-22刺激永生化角质形成细胞株HaCaT细胞增殖、迁移及分化的影响。方法:WST-1法检测紫草素对II-22刺激HaCaT细胞增殖的作用;Transwell法检测紫草素对IL-22刺激HaCaT细胞迁移的影响;Western blot法检测紫草素对IL-22刺激后HaCaT细胞分化相关蛋白兜甲蛋白(loricrin)表达的影响。结果:紫草素可抑制IL-22引起的HaCaT细胞增殖和移动;促进了IL-22刺激后HaCaT细胞分化相关蛋白的表达。结论:紫草素对IL-22介导的体外培养HaCaT细胞增殖及迁移有抑制作用并促进细胞分化。Objective： To investigate the effects of shikonin on the proliferation, migration and differentiation of HaCaT cells treated with interleukin （IL）-22. Methods： WST-1 and transwell assays were used to evaluate the inhibitory effects of shikonin on the proliferation and migration of HaCaT cells incubated with IL-22 respectively. The expression of loricrin（a marker protein of the differentiation of keratinocyte） on HaCaT cells was analyzed by Western blot. Results： The proliferation and migration of HaCaT cells induced by IL-22 were significantly inhibited by shikonin, the down-regulated expressions of loricrin on HaCaT cells induced by IL-22 were increased significantly by adding shikonin. Conclusion： In vitro, shikonin could inhibit the proliferation, migration and promote the differentiation of HaCaT cells mediated by IL-22.

关 键 词：紫草素 IL-22 角质形成细胞 

分 类 号：R751[医药卫生—皮肤病学与性病学]