吴茱萸次碱治疗重症急性胰腺炎的实验研究  

Experimental study of therapeutic use of rutecarpine in severe acute pancreatitis

作　　者：谷欢[1] 彭杰[1] 莫吉祥[2] 

机构地区：[1]中南大学湘雅医院消化内科,湖南长沙410008 [2]湖南省湘潭职业技术学院,湖南湘潭411104

出　　处：《中国普通外科杂志》2014年第3期314-319,共6页China Journal of General Surgery

摘　　要：目的:探讨吴茱萸次碱对大鼠重症急性胰腺炎(SAP)的疗效及其机制。方法:50只SD大鼠随机均分为假手术组和4个实验组,实验组大鼠以5%牛磺胆酸钠胰胆管逆行注射制作SAP模型后,分别给予生理盐水、乌司他丁、吴茱萸次碱、乌司他丁+吴茱萸次碱处理。术后24 h,行病理学检查,并检测各组血清淀粉酶活性及血浆与胰腺组织和内皮素1(EF-1)、降钙素基因相关肽(CGRP)水平。结果:除假手术组外,各实验组大鼠均胰腺组织均出现不同程度的胰腺炎病理改变;与假手术组比较,各实验组均出现不同程度的血清淀粉酶活性升高,血浆与胰腺组织ET-1浓度增加,而血浆与胰腺组织CGRP含量在吴茱萸次碱处理组与联合用药组均明显升高(均P<0.05),其余实验组无明显改变(均P>0.05);实验组中,各药物处理组胰腺病变程度、血清淀粉酶活性、血浆与胰腺组织ET-1浓度均较生理盐水处理的模型组明显降低,且均以联合用药组最为明显(均P<0.05)。结论:吴茱萸次碱对大鼠SAP具有治疗作用,其作用可能与增加CGRP水平从而改善胰腺组织微循环有关。Objective： To investigate the therapeutic effect ofrutecarpine on rats with severe acute pancreatitis （SAP） and its mechanism. Methods： Fifty SD rats were equally randomized into sham operation group and 4 experimental groups. Rats in experimental groups underwent retrograde cholangiopancreatic duct injection of 5% sodium taurocholate to induce SAP model, and then were given the treatment with normal saline, ulinastatin, rutecarpine, or ulinastatin plus rutecarpine, respectively. Twenty-four hours after operation in each group, the pathological examination was performed, the serum amylase activity was measured, and the levels of endothelin 1 （ET-1） and calcitonin gene related peptide （CGRP） in the plasma and pancreatic tissue were determined.Results： Except in sham operation group, all the experimental groups exhibited the pathological profiles of pancreatitis of varying degrees in the pancreatic tissue. Compared with sham operation group, the serum amylase activi~, and levels of ET-1 in the plasma and pancreatic tissue in all experimental groups were increased with different degrees, while the contents of CGRP in plasma and pancreatic tissue were significantly increased in rutecarpine treatment group and combination treatment group （all P〈0.05）, and showed no obvious change in the remaining experimental groups （all P〉0.05）. Among experimental groups, the pancreatic damage was less severe, and serum amylase activity as well as the levels of ET-1 in plasma and pancreatic tissue were significantly decreased in the groups that received drug treatment, especially combination drug treatment, compared with the model group treated with saline （all P〈0.05）. Conclusion： Rutecarpine has protective effect against SAP in rats, which may be associated with its increasing CGRP level and thereby improving the microcirculation of pancreatic tissue.

关 键 词：胰腺炎 急性坏死性 吴茱萸次碱 降钙素基因相关肽 大鼠 

分 类 号：R657.51[医药卫生—外科学]