白细胞抗原G基因14bp插入缺失多态与丙型肝炎病毒慢性感染的关联  

Association of the 14bp Insertion /Deletion Polymorphism in Human Leukocyte Antigen-G Gene with Chronic Hepatitis C Virus Infection

作　　者：张淑琼 张华[2] 汪亚玲 李晓非 王丽萍 刘红伟 沈云松[3] 孙明波[2] 姚宇峰[2] 史荔[2] 

机构地区：[1]昆明市第三人民医院,云南昆明650041 [2]中国医学科学院,北京协和医学院,医学生物学研究所,云南省重大传染病疫苗研发重点实验室,云南昆明650118 [3]云南省第一人民医院,云南昆明650032

出　　处：《贵阳医学院学报》2014年第1期35-38,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金基金资助项目(81160197);昆明市卫生局医药卫生科技项目2012

摘　　要：目的:研究人类白细胞抗原G(HLA-G)基因14 bp插入缺失多态与丙型肝炎病毒(hepatitis C virus,HCV)慢性感染的相关性。方法:选取HCV慢性感染者243例(感染组),无血缘关系的汉族健康对照448例(HCV对照组)。采用PCR序列特异引物法检测HLA-G基因第8外显子3'UTR的1个14 bp插入缺失多态,并比较其在HCV慢性感染人群和健康人群中的分布情况。结果:HLA-G基因14 bp插入等位基因频率在对照组中为0.335,在HCV感染组中为0.348;14 bp插入纯合子基因型频率在对照组中为0.112,在HCV感染组中为0.107。无论是基因型频率还是等位基因频率,在HCV感染组和对照组中均无统计学差异。结论:HLA-G基因14 bp插入缺失多态性与汉族人群中HCV感染的发生无遗传易感染性关联。Objective：To study the correlation between 14bp insertion/deletion polymorphism in human leukocyte antigen-G （HLA-G） gene and the chronic hepatitis C virus （HCV） infection.Methods：A total of 243 patients with chronic HCV infection and 448 healthy controls were included.PCR sequence specific primer method was employed to detect the 14bp insertion/deletion polymorphism of 3＇UTR,the 8ths exon in HLA-G gene,and its distribution in patients with chronic HCV infection and controls was compared.Results：The allele frequencies of 14bp insertion were 0.335 and 0.345 in the control group and HCV infection group respectively,while the homozygote genotype frequencies of 14bp insertion were 0.112 and 0.107.There was no difference between the two groups （P 〉0.05）.Conclusions：The 14bp insertion/deletion polymorphism in HLA-G gene is not associated with chronic HCV infection.

关 键 词：肝炎 丙型 慢性 感染 白细胞抗原 基因 14 bp插入缺失多态 关联研究 

分 类 号：R512.63[医药卫生—内科学]