罗哌卡因聚乙二醇-聚乳酸微球对切口痛模型大鼠坐骨神经阻滞的时效  

Long-term effect of sciatic nerve block w ith ropivacaine PEG-PLA microparticles in a rat model of postoperative pain

作　　者：黄海真[1] 胡启雅 丁超[1] 郭亚秋[1] 齐峰[1] 任杰[2] 潘晓军[1] 

机构地区：[1]山东大学齐鲁医院麻醉科,山东济南250012 [2]山东大学附属济南市中心医院麻醉科,山东济南250013

出　　处：《山东大学学报（医学版）》2014年第3期50-55,共6页Journal of Shandong University:Health Sciences

基　　金：山东大学自主科技创新基金(2012JC017);山东省自然科学基金(ZR2012HM097)

摘　　要：目的探讨罗哌卡因聚乙二醇–聚乳酸(Rop-PEG-PLA)微球对切口痛模型大鼠坐骨神经阻滞的长期作用。方法 80只雄性Wistar大鼠随机分为4组(每组20只),分别于坐骨神经周围间隙给予0.9%NaCl溶液0.2 mL(对照组),PEG-PLA微球10 mg(空白微球组),0.5%罗哌卡因0.2 mL(含罗哌卡因1 mg,罗哌卡因组)或RopPEG-PLA微球10 mg(含罗哌卡因1 mg,罗哌卡因微球组),之后均按照Brennan法制作大鼠切口痛模型。观察术前24 h和术后2 h、4 h、8 h、1 d、2 d、3 d、5 d和7 d时大鼠行为学的变化,免疫组织化学方法检测术后2 h和2 d时脊髓背角c-fos的表达,HPLC法检测大鼠血浆中罗哌卡因的浓度。结果对照组行为学及c-fos表达与空白微球组的差异无统计学意义(P>0.05)。术后2 h时,罗哌卡因组、罗哌卡因微球组行为学阈值与对照组相比均增高(P<0.05),c-fos表达均减少(P<0.05)。罗哌卡因微球组与罗哌卡因组相比,其行为学阈值增高时间(3 d vs2 h)及测得血浆药物时间(8 h vs 4 h)均延长,术后2 d时c-fos表达减少(P<0.05)。结论 Rop-PEG-PLA微球可延长切口痛模型大鼠坐骨神经阻滞镇痛时间达3 d。Objective To investigate the efficiency of ropivacaine PEG-PLA microspheres for sciatic nerve block in rats. Methods Eighty Wistar rats were randomly divided into four groups （n --20, each） and were treated with 0.2 mL of 0.9% saline（ control group）, 10 mg of PEG-PLA（ PEG-PLA group）, 0.2 mL of 0.5% ropivacaine（ ropiva- caine group） and 10 mg of 10% Rop-PEG-PLA microspheres（ Rop-PEG-PLA group）, respectively. The incision pain models were produced as described by Brennan. Mechanical thresholds and thermal latencies were measured at 24 h before incision and at 2, 4, 8 h, as well as 1,2, 3, 5 and 7 d after incision. Expression of c-fos protein in the spinal cord was determined by immunohistochemistry at 2 h and 2 d after incision. The ropivacaine concentration in plasma of ropivacaine group and Rop-PEG-PLA group was detected by HPLC analysis. Results Behavioral testing and c-fos expression in PEG-PLA group showed no significant difference compared with those in control group （ P 〉 0.05 ）. The thresholds and latencies in ropivacaine group and Rop-PEG-PLA group were significantly higher than those in control group at 2 h after incision（ P 〈 0.05 ）. Expressions of c-fos protein were suppressed in ropivacaine group and Rop-PEG-PLA group at 2 h after incision, compared with that in control group （ P 〈 0.05 ）. The duration of anti-hyperalgesia effect （ 3 d vs 2 h） and ropivacaine concentration in plasma （ 8 h vs 4 h） in Rop-PEG-PLA group were longer than those in ropivacaine group, and c-fos expression in Rop-PEG-PLA group was lower than that in ropivacaine group at 2 d after incision （ P 〈 0.05 ）. Conclusion Single administration of ropivacaine PEG-PLA microspheres for sciatic nerve block provides effective analgesia for postoperative pain for 3 d.

关 键 词：罗哌卡因 聚乙二醇-聚乳酸 微球 坐骨神经阻滞 C-FOS 

分 类 号：R614[医药卫生—麻醉学]