体内活化血小板表达CD40L及其对内皮细胞表达炎性因子的影响  

CD40L expressed on activated platelets attained in vivo and its effects on expression of inflammatory cytokines in endothelial cells

作　　者：渠晶 赵爱平[1] 丁卜同[2] 陈昀[1] 常亚丽[1] 郭农建[1] 

机构地区：[1]山东大学附属济南市中心医院血液科,山东济南250013 [2]山东大学附属济南市中心医院病理科,山东济南250013

出　　处：《山东大学学报（医学版）》2014年第3期75-78,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨体内活化血小板表面细胞分化抗原40配体(CD40L)对内皮细胞炎性反应的影响。方法应用流式细胞术检测急性心肌梗死患者(AMI组,n=20)和健康人(正常对照组,n=20)血小板表面CD62P、CD40L的表达水平;体外分离AMI组及正常对照组血小板,分别与培养的人脐静脉内皮细胞(HUVECs)共培养,应用流式细胞术及ELISA法检测HUVECs膜性细胞间黏附分子-1(ICAM-1)、E-选择素(E-selectin)和可溶性白介素-8(IL-8)的水平变化。结果 AMI组CD62P和CD40L表达水平较正常对照组显著升高(P<0.01);AMI组血小板作用于HUVECs后,能促进HUVECs表达ICAM-1、E-selectin和IL-8(P均<0.01),应用特异性CD40L单抗可显著抑制HUVECs炎性因子的表达(P<0.01),而正常对照组血小板对HUVECs表达炎性因子无明显影响(P>0.05)。结论 AMI患者体内活化血小板可高表达CD40L,并对内皮细胞表达炎性因子具有诱导作用。Objective To investigate the effects of platelets surface CD40L attained in vivo on the endothelial inflammatory reaction. Methods CD62P and CD40L expressed on platelets were measured by flow cytometry in patients with acute myocardial infraction （ AMI group, n = 20） and healthy people （ normal control group, n = 20）. Platelets were isolated from the two groups, and then were co-incubated with cultured human umbilical vein endothelial cells （HUVECs） respectively. Membrane-bound intercellular adhesion molecule-1 （ ICAM-I ）, E-selectin and soluble interleukin-8 （ IL-8 ） produced by HUVECs were measured with flow cytometry and ELISA respectively. Results The CD62P and CD40L expressions significantly increased in the AMI group compared with those in the normal control group （ P 〈 0.01 ）. Platelets of the AMI group up-regulated the production of ICAM-1, E-selectin and IL-8 in HUVECs （all P 〈0.01）. CD40L-specific monoclonal antibody could prohibit the high expression of inflammatory cytokines in the AMI group （ P 〈 0.01 ）, while the platelets of normal control group had no effect on the induction of inflammatory cytokines （ P 〉 0.05 ）. Conclusion Activated platelets in AMI patients have a high expression of CD40L and induce endothelial cells to express inflammatory cytokines at the same time.

关 键 词：体内活化 血小板 CD40L 急性心肌梗死 内皮 炎症 

分 类 号：R543.5[医药卫生—心血管疾病]