PiggyBac转座子:人类基因编码研究的新工具  

PiggyBac transposon vectors: The new tools of the human gene encoding

作　　者：于正洪[1] 姜恩泽[2] 张杰明[3] 陈龙邦[1] 

机构地区：[1]南京军区南京总医院肿瘤内科,南京医学博士210002 [2]南京大学医学院,南京210008 [3]美国西北大学文理学院,芝加哥60611

出　　处：《医学研究生学报》2014年第2期199-202,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81272260);江苏省六大人才高峰基金(BK2009284)

摘　　要：转座子是一种不依赖于同源性重组即可在宿主基因组中发生基因座位置改变的DNA片段。PiggyBac(PB)转座子是一种可移动的遗传元素,并通过"剪切-黏贴"机制在有效载体和染色体之间调换。在换位过程中,PB转座子识别具体转座子的反向末端重复序列(inverted terminal repeat sequences,ITRS)位于转座子载体的2端,有效地移动原来位置的内容,并整合到TTAA染色体。PB转座子系统强大的活动使在PB载体上位于2个ITRS之间的基因容易地动员到靶基因。PB转座子因其高转座活性,转座安全、稳定与无痕移除的特性而备受关注,在分子医学研究中有较好的应用前景。针对其特性,PiggyBac转座子可被用于基因转移载体、癌基因筛选工具和基因治疗的载体。文中就其在基因编码研究中的应用作一综述。A transposon is a DNA segment which can change its relative position within the genome of a cell. The PiggyBac （PB） transposon is a mobile genetic element that efficiently transposes between vectors and chromosomes via a ＂cut and paste＂ mechanism. During the transposition, the PB transposase recognizes transposon-specific inverted terminal repeat sequences （ITRs） located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. PiggyBac has drawn much attention because of its transposition efficiency, safety and stability. Due to its priorities, PiggyBac can be used as a new genetic vehicle, a new tool for oncogene screening and a new method for gene therapy. PiggyBac has created a new outlook for molecular medicine.

关 键 词：PIGGYBAC 转座子 转基因 

分 类 号：Q343.1[生物学—遗传学]